Reference - Detail
| RRC ID | 50312 |
|---|---|
| Author | Sasakura Y, Yoshida K, Treen N. |
| Title | Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease. |
| Journal | Methods Mol Biol |
| Abstract |
The ascidian Ciona intestinalis is an important model animal for studying developmental mechanisms for constructing the chordate body. Although molecular and embryological techniques for manipulating Ciona genes were developed a long time ago, recent achievements of genome editing in this animal have innovated functional analyses of genes in Ciona. Particularly, knockout of genes in the G0 generation coupled with tissue-specific expression of TALENs enables us to rapidly address gene functions that were difficult using previous methods. |
| Volume | 1630 |
| Pages | 235-245 |
| Published | 2017-1-1 |
| DOI | 10.1007/978-1-4939-7128-2_19 |
| PMID | 28643263 |
| MeSH | Animals Ciona intestinalis / genetics* Electroporation Gene Editing / methods* Gene Knockout Techniques Organ Specificity Transcription Activator-Like Effector Nucleases / metabolism |
| Resource | |
| C.intestinalis / (O.japonicus) | Wild C. int |