論文 - 詳細
| RRC ID | 50329 |
|---|---|
| 著者 | Kawajiri K, Gotoh O, Sogawa K, Tagashira Y, Muramatsu M, Fujii-Kuriyama Y. |
| タイトル | Coding nucleotide sequence of 3-methylcholanthrene-inducible cytochrome P-450d cDNA from rat liver. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
We determined the coding nucleotide sequence of the mRNA for a 3-methylcholanthrene-inducible cytochrome P-450, P-450d, of rat liver by sequence analysis of cloned cDNAs. The predicted amino acid sequence of the cytochrome is composed of 513 amino acids, and its NH2-terminal sequence of 30 amino acids completely coincides with that reported from analysis of the purified cytochrome P-450d. The amino acid composition of the deduced sequence also agrees well with that determined from the purified protein. Computer-aided analysis was carried out to compare the complete primary structures of five species of cytochrome P-450, two molecular species of phenobarbital-inducible rat liver cytochrome P-450 (P-450b and P-450e), phenobarbital-inducible rabbit liver cytochrome P-450LM2, 3-methylcholanthrene-inducible rat liver cytochrome P-450d, and camphor-hydroxylating P-450 of Pseudomonas putida (P-450CAM). It is concluded therefrom that the time of divergence between cytochrome P-450b (P-450e) and P-450d is much earlier than that of branching between phenobarbital-inducible cytochromes P-450 of rat and rabbit. One highly conserved cysteine-containing region that is close to the COOH terminus is found in all of these cytochrome P-450 sequences, indicating that the heme-binding site is the cysteine residue in this region. |
| 巻・号 | 81(6) |
| ページ | 1649-53 |
| 公開日 | 1984-3-1 |
| DOI | 10.1073/pnas.81.6.1649 |
| PMID | 6584898 |
| PMC | PMC344975 |
| MeSH | Amino Acid Sequence Animals Base Sequence Biological Evolution Cytochrome P-450 Enzyme System / genetics* DNA / genetics Enzyme Induction / drug effects Genes Liver / physiology* Methylcholanthrene / pharmacology RNA, Messenger / genetics Rats |
| IF | 9.412 |
| 引用数 | 170 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | P450 1A2 (CYP1A2) cDNA (RDB01244) |