RRC ID 5034
Author Furuhata K, Goto K, Kato Y, Saitou K, Sugiyama J, Hara M, Yoshida S, Fukuyama M.
Title Characteristics of a pink-pigmented bacterium isolated from biofilm in a cooling tower in Tokyo, Japan.
Journal Microbiol. Immunol.
Abstract Strain K-20, a Gram-negative, non-motile, non-spore-forming and strictly aerobic rod, which produces a pale pink pigment, was isolated from biofilm in a cooling tower in Tokyo, Japan. The taxonomic feature of the strain was studied using phenotypic tests and phylogenetic analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was related to Roseomonas gilardii subsp. rosea, Roseomonas gilardii subsp. gilardii, Roseomonas cervicalis and Roseomonas mucosa at 94.3-94.6 sequence similarities. Growth occurred at 25-40 C and pH 5.0-10.0, optimal at 35 C and pH 7.0. Growth did not occur in the presence of >or=2% NaCl. The API 20NE identification system gave a positive result for urease, L-arabinose, potassium gluconate, adipic acid, malic acid and trisodium citrate (API code number 0201465). The predominant fatty acids of strain K-20 were C18:1Delta11 (50.8%) and C16:1 (17.2%). Cells contained ubiquinone 10 (Q-10) as the major quinone and the G+C content was 72.0 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data, it was assumed that strain K-20 (=JCM 14634) is a novel species of the genus Roseomonas.
Volume 51(6)
Pages 637-41
Published 2007
PII JST.JSTAGE/mandi/51.637
PMID 17579276
MeSH Base Composition Base Sequence Biofilms* DNA, Bacterial / chemistry DNA, Bacterial / genetics Fatty Acids / analysis Methylobacteriaceae / classification Methylobacteriaceae / genetics Methylobacteriaceae / isolation & purification* Methylobacteriaceae / metabolism Microbial Sensitivity Tests Molecular Sequence Data Phylogeny Pigmentation Polymerase Chain Reaction RNA, Ribosomal, 16S / chemistry RNA, Ribosomal, 16S / genetics Sequence Alignment Tokyo Water Microbiology*
IF 1.335
Times Cited 8
General Microbes JCM14634