RRC ID 50371
Author Komatsu K, Masubuchi S.
Title Mouse oocytes connect with granulosa cells by fusing with cell membranes and form a large complex during follicle development.
Journal Biol Reprod
Abstract Proper development and maturation of oocytes requires interaction with granulosa cells. Previous reports have indicated that mammalian oocytes connect with cumulus cells through gap junctions at the tip of transzonal projections that extend from the cells. Although the gap junctions between oocytes and transzonal projections provide a pathway through which small molecules (<1 kDa) can travel, it is unclear how molecules >1 kDa are transported between the oocytes and cumulus cells. In this study, we presented new connections between oocytes and granulosa cells. The green fluorescein protein Aequorea coerulescens green fluorescein protein (AcGFP1) localizing in oocyte cell membrane, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate and dextran conjugates (10,000 MW) injected into the oocytes, which were unable to pass through gap junctions, were diffused from the oocytes into the surrounding granulosa cells through these connections. These connect an oocyte to the surrounding cumulus and granulosa cells by fusing with the cell membranes and forming a large complex during follicle development. Furthermore, we show two characteristics of these connections during follicle development-the localization of growth and differentiation factor-9 within the connections and the dynamics of the connections at ovulation. This article presents for the first time that mammalian oocytes directly connect to granulosa cells by fusing with the cell membrane, similar to that in Drosophila.
Volume 99(3)
Pages 527-535
Published 2018-9-1
DOI 10.1093/biolre/ioy072
PII 4953411
PMID 29590310
PMC PMC6134206
MeSH Animals Cell Membrane / chemistry Cell Membrane / physiology* Female Fluorescent Antibody Technique Granulosa Cells / ultrastructure* Green Fluorescent Proteins / analysis Green Fluorescent Proteins / metabolism Growth Differentiation Factor 9 / analysis Membrane Fusion / physiology* Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Microscopy, Immunoelectron Oocytes / growth & development Oocytes / ultrastructure* Ovarian Follicle / growth & development* Ovulation Tissue Culture Techniques
IF 3.322
Times Cited 8
Mice RBRC06134