RRC ID 50373
Author Ishii A, Kawai M, Noda H, Kato H, Takeda K, Asakawa K, Ichikawa Y, Sasanami T, Tanaka K, Kimura Y.
Title Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts.
Journal Sci Rep
Abstract When exposed to sublethal high temperatures, budding yeast cells can survive for a period of time; however, a sufficient amount of ubiquitin is necessary for this survival. To understand the nature of the stress, we examined the morphological changes in yeast cells, focusing on the vacuoles. Changes in vacuolar morphology were notable, and ruffled vacuolar membranes, accelerated invaginations of vacuolar membranes, and vesicle-like formations were observed. These changes occurred in the absence of Atg1, Atg9 or Ivy1 but appeared to require endosomal sorting proteins, such as Vps23, Vps24 or Pep12. Furthermore, the serial sections of the vacuoles analysed using an electron microscopic analysis revealed that spherical invaginated structures were linked together in a vacuole. Because degradation of cell surface proteins is induced from heat stress, fusion of endosomal and vacuolar membranes might occur frequently in heat-stressed cells, and yeast cells might be able to cope with a rapid increase in vacuolar surface area by such invaginations.
Volume 8(1)
Pages 2644
Published 2018-2-8
DOI 10.1038/s41598-018-20781-8
PII 10.1038/s41598-018-20781-8
PMID 29422608
PMC PMC5805771
MeSH Autophagy-Related Proteins / metabolism Carrier Proteins / genetics Carrier Proteins / metabolism Endosomes / metabolism Heat-Shock Response / genetics Heat-Shock Response / physiology* Hot Temperature Membrane Proteins / genetics Membrane Proteins / metabolism Microscopy, Electron Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae / ultrastructure Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Vacuoles / physiology* Vacuoles / ultrastructure
IF 3.998
Times Cited 1
Yeast BY20695