| RRC ID |
50382
|
| Author |
Sugihara A, Nguyen LC, Shamim HM, Iida T, Nakase M, Takegawa K, Senda M, Jida S, Ueno M.
|
| Title |
Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Fission yeast Pik1p is one of three phosphatidylinositol 4-kinases associated with the Golgi complex, but its function is not fully understood. Deletion of pot1+ causes telomere degradation and chromosome circularization. We searched for the gene which becomes synthetically lethal with pot1Δ. We obtained a novel pik1 mutant, pik1-1, which is synthetically lethal with pot1Δ. We found phosphoinositol 4-phosphate in the Golgi was reduced in pik1-1. To investigate the mechanism of the lethality of the pot1Δ pik1-1 double mutant, we constructed the nmt-pot1-aid pik1-1 strain, where Pot1 function becomes low by drugs, which leads to telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1+ is required for the maintenance of circular chromosomes.
|
| Volume |
496(4)
|
| Pages |
1284-1290
|
| Published |
2018-2-19
|
| DOI |
10.1016/j.bbrc.2018.02.001
|
| PII |
S0006-291X(18)30222-5
|
| PMID |
29410177
|
| MeSH |
Apoptosis / genetics*
Mutation / genetics*
Phosphoproteins / genetics*
Saccharomyces cerevisiae Proteins / genetics*
Schizosaccharomyces / cytology
Schizosaccharomyces / physiology*
Schizosaccharomyces pombe Proteins / genetics*
Telomere / genetics*
|
| IF |
2.985
|
| Times Cited |
0
|
| Resource |
| Yeast |
|
