論文 - 詳細
| RRC ID | 50391 |
|---|---|
| 著者 | Kuwana R, Okuda N, Takamatsu H, Watabe K. |
| タイトル | Modification of GerQ reveals a functional relationship between Tgl and YabG in the coat of Bacillus subtilis spores. |
| ジャーナル | J Biochem |
| Abstract |
Here we describe the functional relationship between YabG and transglutaminase (Tgl), enzymes that modify the spore coat proteins of Bacillus subtilis. In wild-type spores at 37 degrees C, Tgl mediates the crosslinking of GerQ into higher molecular mass forms; however, some GerQ multimers are found in tgl mutant spores, indicating that Tgl is not essential. Immunoblotting showed that spores isolated from a yabG mutant after sporulation at 37 degrees C contain only very low levels of GerQ multimers. Heat treatment for 20 min at 60 degrees C, which maximally activates the enzymatic activity of Tgl, caused crosslinking of GerQ in isolated yabG spores but not in tgl/yabG double-mutant spores. In addition, the germination frequency of the tgl/yabG spores in the presence of l-alanine with or without heat activation at 60 degrees C was lower than that of wild-type spores. These findings suggest that Tgl cooperates with YabG to mediate the temperature-dependent modification of the coat proteins, a process associated with spore germination in B. subtilis. |
| 巻・号 | 139(5) |
| ページ | 887-901 |
| 公開日 | 2006-5-1 |
| DOI | 10.1093/jb/mvj096 |
| PII | 139/5/887 |
| PMID | 16751597 |
| MeSH | Ammonium Sulfate / pharmacology Bacillus subtilis / genetics Bacillus subtilis / metabolism* Bacterial Proteins / drug effects Bacterial Proteins / metabolism* Endopeptidases / genetics Endopeptidases / metabolism Hot Temperature Hydrolases / metabolism Sigma Factor / metabolism Spores, Bacterial / enzymology* Spores, Bacterial / genetics* Spores, Bacterial / growth & development Transcription Factors / metabolism* Transglutaminases / genetics Transglutaminases / metabolism* |
| IF | 2.476 |
| 引用数 | 27 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(枯草菌) | MBS567 |