論文 - 詳細
| RRC ID | 50414 |
|---|---|
| 著者 | Szostak E, García-Beyaert M, Guitart T, Graindorge A, Coll O, Gebauer F. |
| タイトル | Hrp48 and eIF3d contribute to msl-2 mRNA translational repression. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Translational repression of msl-2 mRNA in females of Drosophila melanogaster is an essential step in the regulation of X-chromosome dosage compensation. Repression is orchestrated by Sex-lethal (SXL), which binds to both untranslated regions (UTRs) of msl-2 and inhibits translation initiation by poorly understood mechanisms. Here we identify Hrp48 as a SXL co-factor. Hrp48 binds to the 3' UTR of msl-2 and is required for optimal repression by SXL. Hrp48 interacts with eIF3d, a subunit of the eIF3 translation initiation complex. Reporter and RNA chromatography assays showed that eIF3d binds to msl-2 5' UTR, and is required for efficient translation and translational repression of msl-2 mRNA. In line with these results, eIF3d depletion -but not depletion of other eIF3 subunits- de-represses msl-2 expression in female flies. These data are consistent with a model where Hrp48 inhibits msl-2 translation by targeting eIF3d. Our results uncover an important step in the mechanism of msl-2 translation regulation, and illustrate how general translation initiation factors can be co-opted by RNA binding proteins to achieve mRNA-specific control. |
| 巻・号 | 46(8) |
| ページ | 4099-4113 |
| 公開日 | 2018-5-4 |
| DOI | 10.1093/nar/gky246 |
| PII | 4959919 |
| PMID | 29635389 |
| PMC | PMC5934621 |
| MeSH | 5' Untranslated Regions Animals DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism* Drosophila Proteins / physiology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Eukaryotic Initiation Factor-3 / antagonists & inhibitors Eukaryotic Initiation Factor-3 / metabolism* Female Gene Expression Regulation* Heterogeneous-Nuclear Ribonucleoproteins / metabolism* Heterogeneous-Nuclear Ribonucleoproteins / physiology Nuclear Proteins / genetics* Nuclear Proteins / metabolism Protein Biosynthesis* Ribonucleoproteins / metabolism Transcription Factors / genetics* Transcription Factors / metabolism |
| IF | 11.502 |
| 引用数 | 5 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 10161R-3 |