RRC ID 50415
Author Wang H, Qiu Z, Xu Z, Chen SJ, Luo J, Wang X, Chen J.
Title aPKC is a key polarity determinant in coordinating the function of three distinct cell polarities during collective migration.
Journal Development
Abstract Apical-basal polarity is a hallmark of epithelia and needs to be remodeled when epithelial cells undergo morphogenetic cell movements. Here, we analyze border cells in the Drosophila ovary to address how apical-basal polarity is remodeled and turned into front-back and inside-outside as well as apical-basal polarities, during collective migration. We find that the Crumbs (Crb) complex is required for the generation of the three distinct but interconnected cell polarities of border cells. Specifically, the Crb complex, together with the Par complex and the endocytic recycling machinery, ensures the strict distribution of two distinct populations of aPKC at the inside apical junction and near the outside lateral membrane. Interestingly, aPKC distributed near the outside lateral membrane interacts with Sif and promotes Rac-induced protrusions, whereas alteration of the aPKC distribution pattern changes the pattern of protrusion formation, leading to disruption of all three polarities. Therefore, we demonstrate that aPKC, spatially controlled by the Crb complex, is a key polarity molecule coordinating the generation of three distinct but interconnected cell polarities during collective migration.
Volume 145(9)
Published 2018-5-2
DOI 10.1242/dev.158444
PII dev.158444
PMID 29636381
MeSH Animals Cell Membrane / genetics Cell Membrane / metabolism* Cell Movement / physiology* Cell Polarity / physiology* Drosophila Proteins / genetics Drosophila Proteins / metabolism Drosophila melanogaster Epithelial Cells / cytology Epithelial Cells / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Morphogenesis / physiology* Protein Kinase C / genetics Protein Kinase C / metabolism*
IF 5.763
Times Cited 10
Resource
Drosophila 12021R-3 15342R-2 5055R-1 5055R-2 5406R-2 5406R-3 12530R-2 12530R-3