RRC ID |
50431
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著者 |
Nguyen GN, Yamagata Y, Shigematsu Y, Watanabe M, Miyazaki Y, Doi K, Tashiro K, Kuhara S, Kanamori H, Wu J, Matsumoto T, Yasui H, Yoshimura A.
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タイトル |
Duplication and Loss of Function of Genes Encoding RNA Polymerase III Subunit C4 Causes Hybrid Incompatibility in Rice.
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ジャーナル |
G3 (Bethesda)
|
Abstract |
Reproductive barriers are commonly observed in both animals and plants, in which they maintain species integrity and contribute to speciation. This report shows that a combination of loss-of-function alleles at two duplicated loci, DUPLICATED GAMETOPHYTIC STERILITY 1 (DGS1) on chromosome 4 and DGS2 on chromosome 7, causes pollen sterility in hybrid progeny derived from an interspecific cross between cultivated rice, Oryza sativa, and an Asian annual wild rice, O. nivara Male gametes carrying the DGS1 allele from O. nivara (DGS1-nivaras ) and the DGS2 allele from O. sativa (DGS2-T65s ) were sterile, but female gametes carrying the same genotype were fertile. We isolated the causal gene, which encodes a protein homologous to DNA-dependent RNA polymerase (RNAP) III subunit C4 (RPC4). RPC4 facilitates the transcription of 5S rRNAs and tRNAs. The loss-of-function alleles at DGS1-nivaras and DGS2-T65s were caused by weak or nonexpression of RPC4 and an absence of RPC4, respectively. Phylogenetic analysis demonstrated that gene duplication of RPC4 at DGS1 and DGS2 was a recent event that occurred after divergence of the ancestral population of Oryza from other Poaceae or during diversification of AA-genome species.
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巻・号 |
7(8)
|
ページ |
2565-2575
|
公開日 |
2017-8-7
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DOI |
10.1534/g3.117.043943
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PII |
g3.117.043943
|
PMID |
28592558
|
PMC |
PMC5555463
|
MeSH |
Chromosome Mapping
Chromosome Segregation / genetics
Cloning, Molecular
Crosses, Genetic
Epistasis, Genetic
Fertility / genetics
Gene Duplication*
Gene Expression Regulation, Plant
Genes, Plant*
Genetic Linkage
Genotype
Germination / genetics
Heterozygote
Hybridization, Genetic*
Oryza / enzymology*
Oryza / genetics*
Plant Infertility / genetics
Plant Proteins / genetics*
Plant Proteins / metabolism
Pollen / genetics
Protein Subunits / genetics*
Protein Subunits / metabolism
RNA Polymerase III / genetics*
RNA Polymerase III / metabolism
Time Factors
|
IF |
2.781
|
引用数 |
14
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リソース情報 |
イネ |
CSSL |