RRC ID 50472
著者 Belu M, Mizutani CM.
タイトル Variation in mesoderm specification across Drosophilids is compensated by different rates of myoblast fusion during body wall musculature development.
ジャーナル PLoS One
Abstract BACKGROUND:It has been shown that species separated by relatively short evolutionary distances may have extreme variations in egg size and shape. Those variations are expected to modify the polarized morphogenetic gradients that pattern the dorso-ventral axis of embryos. Currently, little is known about the effects of scaling over the embryonic architecture of organisms. We began examining this problem by asking if changes in embryo size in closely related species of Drosophila modify all three dorso-ventral germ layers or only particular layers, and whether or not tissue patterning would be affected at later stages.
PRINCIPAL FINDINGS:Here we report that changes in scale affect predominantly the mesodermal layer at early stages, while the neuroectoderm remains constant across the species studied. Next, we examined the fate of somatic myoblast precursor cells that derive from the mesoderm to test whether the assembly of the larval body wall musculature would be affected by the variation in mesoderm specification. Our results show that in all four species analyzed, the stereotyped organization of the body wall musculature is not disrupted and remains the same as in D. melanogaster. Instead, the excess or shortage of myoblast precursors is compensated by the formation of individual muscle fibers containing more or less fused myoblasts.
CONCLUSIONS:Our data suggest that changes in embryonic scaling often lead to expansions or retractions of the mesodermal domain across Drosophila species. At later stages, two compensatory cellular mechanisms assure the formation of a highly stereotyped larval somatic musculature: an invariable selection of 30 muscle founder cells per hemisegment, which seed the formation of a complete array of muscle fibers, and a variable rate in myoblast fusion that modifies the number of myoblasts that fuse to individual muscle fibers.
巻・号 6(12)
ページ e28970
公開日 2011-12-14
DOI 10.1371/journal.pone.0028970
PII PONE-D-11-16662
PMID 22194964
PMC PMC3237579
MeSH Animals Axons / metabolism Biological Evolution Blastoderm / cytology Blastoderm / embryology Body Patterning* Body Size Cell Count Cell Fusion Cell Nucleus / metabolism Drosophilidae / anatomy & histology* Drosophilidae / cytology Drosophilidae / embryology* Drosophilidae / genetics Embryo, Nonmammalian / anatomy & histology Embryo, Nonmammalian / cytology Embryo, Nonmammalian / innervation Gene Expression Regulation, Developmental Insect Proteins / genetics Insect Proteins / metabolism Larva / growth & development Larva / metabolism Mesoderm / cytology Mesoderm / embryology* Motor Neurons / cytology Motor Neurons / metabolism Muscle Fibers, Skeletal / cytology Muscle Fibers, Skeletal / metabolism Muscles / embryology* Muscles / innervation Myoblasts / cytology* Myoblasts / metabolism Species Specificity
IF 2.74
引用数 3
リソース情報
ショウジョウバエ D. pseudoobscura (K-s12)