論文 - 詳細
| RRC ID | 50476 |
|---|---|
| 著者 | Hashimoto Y, Tabuchi Y, Sakurai K, Kutsuna M, Kurokawa K, Awasaki T, Sekimizu K, Nakanishi Y, Shiratsuchi A. |
| タイトル | Identification of lipoteichoic acid as a ligand for draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes. |
| ジャーナル | J Immunol |
| Abstract |
Phagocytosis is central to cellular immunity against bacterial infections. As in mammals, both opsonin-dependent and -independent mechanisms of phagocytosis seemingly exist in Drosophila. Although candidate Drosophila receptors for phagocytosis have been reported, how they recognize bacteria, either directly or indirectly, remains to be elucidated. We searched for the Staphylococcus aureus genes required for phagocytosis by Drosophila hemocytes in a screening of mutant strains with defects in the structure of the cell wall. The genes identified included ltaS, which encodes an enzyme responsible for the synthesis of lipoteichoic acid. ltaS-dependent phagocytosis of S. aureus required the receptor Draper but not Eater or Nimrod C1, and Draper-lacking flies showed reduced resistance to a septic infection of S. aureus without a change in a humoral immune response. Finally, lipoteichoic acid bound to the extracellular region of Draper. We propose that lipoteichoic acid serves as a ligand for Draper in the phagocytosis of S. aureus by Drosophila hemocytes and that the phagocytic elimination of invading bacteria is required for flies to survive the infection. |
| 巻・号 | 183(11) |
| ページ | 7451-60 |
| 公開日 | 2009-12-1 |
| DOI | 10.4049/jimmunol.0901032 |
| PII | jimmunol.0901032 |
| PMID | 19890048 |
| MeSH | Animals Drosophila / immunology* Drosophila / microbiology Drosophila Proteins / immunology* Drosophila Proteins / metabolism Hemocytes / immunology* Hemocytes / metabolism Hemocytes / microbiology Ligands Lipopolysaccharides / genetics Lipopolysaccharides / immunology Lipopolysaccharides / metabolism* Membrane Proteins / immunology* Membrane Proteins / metabolism Phagocytosis / physiology* Reverse Transcriptase Polymerase Chain Reaction Staphylococcal Infections / immunology* Staphylococcal Infections / metabolism Staphylococcus aureus / genetics Staphylococcus aureus / immunology Teichoic Acids / genetics Teichoic Acids / immunology Teichoic Acids / metabolism* |
| IF | 4.718 |
| 引用数 | 52 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| ショウジョウバエ | Oregon R |