RRC ID 50476
著者 Hashimoto Y, Tabuchi Y, Sakurai K, Kutsuna M, Kurokawa K, Awasaki T, Sekimizu K, Nakanishi Y, Shiratsuchi A.
タイトル Identification of lipoteichoic acid as a ligand for draper in the phagocytosis of Staphylococcus aureus by Drosophila hemocytes.
ジャーナル J Immunol
Abstract Phagocytosis is central to cellular immunity against bacterial infections. As in mammals, both opsonin-dependent and -independent mechanisms of phagocytosis seemingly exist in Drosophila. Although candidate Drosophila receptors for phagocytosis have been reported, how they recognize bacteria, either directly or indirectly, remains to be elucidated. We searched for the Staphylococcus aureus genes required for phagocytosis by Drosophila hemocytes in a screening of mutant strains with defects in the structure of the cell wall. The genes identified included ltaS, which encodes an enzyme responsible for the synthesis of lipoteichoic acid. ltaS-dependent phagocytosis of S. aureus required the receptor Draper but not Eater or Nimrod C1, and Draper-lacking flies showed reduced resistance to a septic infection of S. aureus without a change in a humoral immune response. Finally, lipoteichoic acid bound to the extracellular region of Draper. We propose that lipoteichoic acid serves as a ligand for Draper in the phagocytosis of S. aureus by Drosophila hemocytes and that the phagocytic elimination of invading bacteria is required for flies to survive the infection.
巻・号 183(11)
ページ 7451-60
公開日 2009-12-1
DOI 10.4049/jimmunol.0901032
PII jimmunol.0901032
PMID 19890048
MeSH Animals Drosophila / immunology* Drosophila / microbiology Drosophila Proteins / immunology* Drosophila Proteins / metabolism Hemocytes / immunology* Hemocytes / metabolism Hemocytes / microbiology Ligands Lipopolysaccharides / genetics Lipopolysaccharides / immunology Lipopolysaccharides / metabolism* Membrane Proteins / immunology* Membrane Proteins / metabolism Phagocytosis / physiology* Reverse Transcriptase Polymerase Chain Reaction Staphylococcal Infections / immunology* Staphylococcal Infections / metabolism Staphylococcus aureus / genetics Staphylococcus aureus / immunology Teichoic Acids / genetics Teichoic Acids / immunology Teichoic Acids / metabolism*
IF 4.718
引用数 52
リソース情報
ショウジョウバエ Oregon R