RRC ID 5048
Author Kaksonen AH, Spring S, Schumann P, Kroppenstedt RM, Puhakka JA.
Title Desulfurispora thermophila gen. nov., sp. nov., a thermophilic, spore-forming sulfate-reducer isolated from a sulfidogenic fluidized-bed reactor.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a sulfidogenic fluidized-bed reactor treating acidic metal- and sulfate-containing water. The strain, designated RA50E1(T), was rod-shaped and motile. The strain grew at 40-67 degrees C (optimum growth at 59-61 degrees C) and pH 6.4-7.9 (optimum growth at pH 7.0-7.3). The strain tolerated up to 1 % NaCl. Sulfate, sulfite, thiosulfate and elemental sulfur were used as electron acceptors, but not nitrate, nitrite or iron(III). Electron donors utilized were H(2)/CO(2) (80 : 20, v/v), alcohols, various carboxylic acids and some sugars. Fermentative growth occurred on lactate and pyruvate. The cell wall contained meso-diaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone MK-7. Major whole-cell fatty acids were iso-C(15 : 0) and iso-C(17 : 0). Strain RA50E1(T) was distantly related to representatives of the genera Desulfotomaculum, Pelotomaculum, Sporotomaculum and Cryptanaerobacter. On the basis of 16S rRNA gene sequence data, the strain cannot be assigned to any known genus. Based on the phenotypic and phylogenetic features of strain RA50E1(T), it is proposed that the strain represents a novel species in a new genus, for which the name Desulfurispora thermophila gen. nov., sp. nov. is proposed. The type strain of Desulfurispora thermophila is RA50E1(T) (=DSM 16022(T)=JCM 14018(T)).
Volume 57(Pt 5)
Pages 1089-1094
Published 2007-5
DOI 10.1099/ijs.0.64593-0
PMID 17473265
MeSH Alcohols / metabolism Bacterial Typing Techniques Carbohydrate Metabolism Carbon Dioxide / metabolism Carboxylic Acids / metabolism Cell Wall / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Diaminopimelic Acid / isolation & purification Fatty Acids / analysis Genes, rRNA Gram-Positive Endospore-Forming Rods / chemistry Gram-Positive Endospore-Forming Rods / classification* Gram-Positive Endospore-Forming Rods / genetics Gram-Positive Endospore-Forming Rods / isolation & purification Gram-Positive Endospore-Forming Rods / metabolism* Hydrogen / metabolism Hydrogen-Ion Concentration Microscopy, Phase-Contrast Molecular Sequence Data Movement Oxidation-Reduction Phylogeny Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Sulfates / metabolism* Sulfites / metabolism Sulfur / metabolism Temperature Thiosulfates / metabolism Water Microbiology*
IF 2.166
Times Cited 12
WOS Category MICROBIOLOGY
Resource
General Microbes JCM14018