RRC ID 50521
Author Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM.
Title mKikGR, a monomeric photoswitchable fluorescent protein.
Journal PLoS One
Abstract The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging.
Volume 3(12)
Pages e3944
Published 2008-1-1
DOI 10.1371/journal.pone.0003944
PMID 19079591
PMC PMC2592542
MeSH Animals Anthozoa / chemistry* Kinetics Light* Luminescent Proteins / chemistry Luminescent Proteins / metabolism* Photobleaching / radiation effects Spectrometry, Fluorescence
IF 2.776
Times Cited 122
Resource
DNA material mKikGR/pRSETB (RDB15261).