論文 - 詳細
| RRC ID | 50521 |
|---|---|
| 著者 | Habuchi S, Tsutsui H, Kochaniak AB, Miyawaki A, van Oijen AM. |
| タイトル | mKikGR, a monomeric photoswitchable fluorescent protein. |
| ジャーナル | PLoS One |
| Abstract |
The recent demonstration and utilization of fluorescent proteins whose fluorescence can be switched on and off has greatly expanded the toolkit of molecular and cell biology. These photoswitchable proteins have facilitated the characterization of specifically tagged molecular species in the cell and have enabled fluorescence imaging of intracellular structures with a resolution far below the classical diffraction limit of light. Applications are limited, however, by the fast photobleaching, slow photoswitching, and oligomerization typical for photoswitchable proteins currently available. Here, we report the molecular cloning and spectroscopic characterization of mKikGR, a monomeric version of the previously reported KikGR that displays high photostability and switching rates. Furthermore, we present single-molecule imaging experiments that demonstrate that individual mKikGR proteins can be localized with a precision of better than 10 nanometers, suggesting their suitability for super-resolution imaging. |
| 巻・号 | 3(12) |
| ページ | e3944 |
| 公開日 | 2008-1-1 |
| DOI | 10.1371/journal.pone.0003944 |
| PMID | 19079591 |
| PMC | PMC2592542 |
| MeSH | Animals Anthozoa / chemistry* Kinetics Light* Luminescent Proteins / chemistry Luminescent Proteins / metabolism* Photobleaching / radiation effects Spectrometry, Fluorescence |
| IF | 2.74 |
| 引用数 | 122 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 20 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | mKikGR/pRSETB (RDB15261) CoralHue™ humanized monomeric Kikume Green-Red 1 (phmKikGR1-MNL) (RDB18303) CoralHue™ monomeric Kikume Green-Red 1 (pmKikGR1-S1) (RDB18304) CoralHue™ humanized monomeric Kikume Green-Red 1 (phmKikGR1-MCL) (RDB18305) |