論文 - 詳細
| RRC ID | 50559 |
|---|---|
| 著者 | Ishikawa T, Kashima M, Nagano AJ, Ishikawa-Fujiwara T, Kamei Y, Todo T, Mori K. |
| タイトル | Unfolded protein response transducer IRE1-mediated signaling independent of XBP1 mRNA splicing is not required for growth and development of medaka fish. |
| ジャーナル | Elife |
| Abstract |
When activated by the accumulation of unfolded proteins in the endoplasmic reticulum, metazoan IRE1, the most evolutionarily conserved unfolded protein response (UPR) transducer, initiates unconventional splicing of XBP1 mRNA. Unspliced and spliced mRNA are translated to produce pXBP1(U) and pXBP1(S), respectively. pXBP1(S) functions as a potent transcription factor, whereas pXBP1(U) targets pXBP1(S) to degradation. In addition, activated IRE1 transmits two signaling outputs independent of XBP1, namely activation of the JNK pathway, which is initiated by binding of the adaptor TRAF2 to phosphorylated IRE1, and regulated IRE1-dependent decay (RIDD) of various mRNAs in a relatively nonspecific manner. Here, we conducted comprehensive and systematic genetic analyses of the IRE1-XBP1 branch of the UPR using medaka fish and found that the defects observed in XBP1-knockout or IRE1-knockout medaka were fully rescued by constitutive expression of pXBP1(S). Thus, the JNK and RIDD pathways are not required for the normal growth and development of medaka. The unfolded protein response sensor/transducer IRE1-mediated splicing of XBP1 mRNA encoding its active downstream transcription factor to maintain the homeostasis of the endoplasmic reticulum is sufficient for growth and development of medaka fish. |
| 巻・号 | 6 |
| 公開日 | 2017-9-27 |
| DOI | 10.7554/eLife.26845 |
| PII | 26845 |
| PMID | 28952924 |
| PMC | PMC5636610 |
| MeSH | Animals Endoribonucleases / genetics Endoribonucleases / metabolism* Gene Knockout Techniques Genetic Complementation Test Oryzias / growth & development* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* RNA Splicing* Signal Transduction* Unfolded Protein Response* X-Box Binding Protein 1 / genetics X-Box Binding Protein 1 / metabolism* |
| IF | 7.08 |
| 引用数 | 14 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | OK-Cab (MT830) Tg(PBiP-EGFP) (TG998) LCE/GFP (TG133) TILLING Mutant |