Reference - Detail
| RRC ID | 5057 |
|---|---|
| Author | Hua NP, Kanekiyo A, Fujikura K, Yasuda H, Naganuma T. |
| Title | Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria isolated from a deep-sea methane cold seep. |
| Journal | Int J Syst Evol Microbiol |
| Abstract |
Two Gram-positive, rod-shaped, moderately halophilic bacteria were isolated from a deep-sea carbonate rock at a methane cold seep in Kuroshima Knoll, Japan. These bacteria, strains IS-Hb4(T) and IS-Hb7(T), were spore-forming and non-motile. They were able to grow at temperatures as low as 9 degrees C and hydrostatic pressures up to 30 MPa. Based on high sequence similarity of their 16S rRNA genes to those of type strains of the genus Halobacillus, from 96.4 % (strain IS-Hb7(T) to Halobacillus halophilus NCIMB 9251(T)) to 99.4 % (strain IS-Hb4(T) to Halobacillus dabanensis D-8(T)), the strains were shown to belong to this genus. DNA-DNA relatedness values of 49.5 % and 1.0-33.0 %, respectively, were determined between strains IS-Hb4(T) and IS-Hb7(T) and between these strains and other Halobacillus type strains. Both strains showed the major menaquinone MK7 and L-orn-D-Asp cell-wall peptidoglycan type. Straight-chain C(16 : 0), unsaturated C(16 : 1)omega7c alcohol and C(18 : 1)omega7c and cyclopropane C(19 : 0) cyc fatty acids were predominant in both strains. The DNA G+C contents of IS-Hb4(T) and IS-Hb7(T) were respectively 43.3 and 42.1 mol%. Physiological and biochemical analyses combined with DNA-DNA hybridization results allowed us to place strains IS-Hb4(T) (=JCM 14154(T)=DSM 18394(T)) and IS-Hb7(T) (=JCM 14155(T)=DSM 18393(T)) in the genus Halobacillus as the respective type strains of the novel species Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov. |
| Volume | 57(Pt 6) |
| Pages | 1243-1249 |
| Published | 2007-6-1 |
| DOI | 10.1099/ijs.0.64817-0 |
| PMID | 17551037 |
| MeSH | Bacillaceae / classification* Bacillaceae / cytology Bacillaceae / isolation & purification* Bacillaceae / physiology Bacterial Typing Techniques Base Composition Cold Temperature DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Genes, rRNA Hydrostatic Pressure Japan Locomotion Microscopy, Phase-Contrast Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Sequence Homology, Nucleic Acid Spores, Bacterial |
| IF | 2.415 |
| Times Cited | 29 |
| WOS Category | MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM14154 JCM14155 JCM11546 |