| RRC ID |
50583
|
| 著者 |
Prado Acosta M, Ruzal SM, Cordo SM.
|
| タイトル |
S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor.
|
| ジャーナル |
Int J Biol Macromol
|
| Abstract |
Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells.
|
| 巻・号 |
92
|
| ページ |
998-1005
|
| 公開日 |
2016-11-1
|
| DOI |
10.1016/j.ijbiomac.2016.07.096
|
| PII |
S0141-8130(16)31026-1
|
| PMID |
27498415
|
| MeSH |
3T3 Cells
Animals
Anti-Bacterial Agents / pharmacology
Bacterial Adhesion / drug effects
Bacterial Infections / drug therapy*
Cell Adhesion Molecules / metabolism*
Glycosylation / drug effects
Humans
Lactobacillus / chemistry*
Lectins, C-Type / metabolism*
Membrane Glycoproteins / pharmacology
Membrane Glycoproteins / therapeutic use*
Mice
Receptors, Cell Surface / metabolism*
|
| IF |
5.162
|
| 引用数 |
18
|
| リソース情報 |
| 一般微生物 |
JCM 5818 |
