論文 - 詳細
| RRC ID | 50583 |
|---|---|
| 著者 | Prado Acosta M, Ruzal SM, Cordo SM. |
| タイトル | S-layer proteins from Lactobacillus sp. inhibit bacterial infection by blockage of DC-SIGN cell receptor. |
| ジャーナル | Int J Biol Macromol |
| Abstract |
Many species of Lactobacillus sp. possess Surface(s) layer proteins in their envelope. Among other important characteristics S-layer from Lactobacillus acidophilus binds to the cellular receptor DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin; CD209), which is involved in adhesion and infection of several families of bacteria. In this report we investigate the activity of new S-layer proteins from the Lactobacillus family (Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus helveticus and Lactobacillus kefiri) over the infection of representative microorganisms important to human health. After the treatment of DC-SIGN expressing cells with these proteins, we were able to diminish bacterial infection by up to 79% in both gram negative and mycobacterial models. We discovered that pre-treatment of the bacteria with S-layers from Lactobacillus acidophilus and Lactobacillus brevis reduced bacteria viability but also prevent infection by the pathogenic bacteria. We also proved the importance of the glycosylation of the S-layer from Lactobacillus kefiri in the binding to the receptor and thus inhibition of infection. This novel characteristic of the S-layers proteins may contribute to the already reported pathogen exclusion activity for these Lactobacillus probiotic strains; and might be also considered as a novel enzymatic antimicrobial agents to inhibit bacterial infection and entry to host cells. |
| 巻・号 | 92 |
| ページ | 998-1005 |
| 公開日 | 2016-11-1 |
| DOI | 10.1016/j.ijbiomac.2016.07.096 |
| PII | S0141-8130(16)31026-1 |
| PMID | 27498415 |
| MeSH | 3T3 Cells Animals Anti-Bacterial Agents / pharmacology Bacterial Adhesion / drug effects Bacterial Infections / drug therapy* Cell Adhesion Molecules / metabolism* Glycosylation / drug effects Humans Lactobacillus / chemistry* Lectins, C-Type / metabolism* Membrane Glycoproteins / pharmacology Membrane Glycoproteins / therapeutic use* Mice Receptors, Cell Surface / metabolism* |
| IF | 5.162 |
| 引用数 | 18 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5818 |