論文 - 詳細
| RRC ID | 50593 |
|---|---|
| 著者 | Kashyap DR, Kuzma M, Kowalczyk DA, Gupta D, Dziarski R. |
| タイトル | Bactericidal peptidoglycan recognition protein induces oxidative stress in Escherichia coli through a block in respiratory chain and increase in central carbon catabolism. |
| ジャーナル | Mol Microbiol |
| Abstract |
Mammalian Peptidoglycan Recognition Proteins (PGRPs) kill both Gram-positive and Gram-negative bacteria through simultaneous induction of oxidative, thiol and metal stress responses in bacteria. However, metabolic pathways through which PGRPs induce these bactericidal stress responses are unknown. We screened Keio collection of Escherichia coli deletion mutants and revealed that deleting genes for respiratory chain flavoproteins or for tricarboxylic acid (TCA) cycle resulted in increased resistance of E. coli to PGRP killing. PGRP-induced killing depended on the production of hydrogen peroxide, which required increased supply of NADH for respiratory chain oxidoreductases from central carbon catabolism (glycolysis and TCA cycle), and was controlled by cAMP-Crp. Bactericidal PGRP induced a rapid decrease in respiration, which suggested that the main source of increased production of hydrogen peroxide was a block in respiratory chain and diversion of electrons from NADH oxidoreductases to oxygen. CpxRA two-component system was a negative regulator of PGRP-induced oxidative stress. By contrast, PGRP-induced thiol stress (depletion of thiols) and metal stress (increase in intracellular free Zn2+ through influx of extracellular Zn2+ ) were mostly independent of oxidative stress. Thus, manipulating pathways that induce oxidative, thiol and metal stress in bacteria could be a useful strategy to design new approaches to antibacterial therapy. |
| 巻・号 | 105(5) |
| ページ | 755-776 |
| 公開日 | 2017-9-1 |
| DOI | 10.1111/mmi.13733 |
| PMID | 28621879 |
| PMC | PMC5570643 |
| MeSH | Anti-Bacterial Agents / metabolism* Carbon / metabolism Carrier Proteins / immunology Carrier Proteins / metabolism* Citric Acid Cycle Electron Transport Escherichia coli / metabolism Escherichia coli Proteins / metabolism Flavoproteins Gram-Negative Bacteria / metabolism Gram-Positive Bacteria / metabolism Immunity, Innate Oxidative Stress / physiology Peptidoglycan / metabolism* |
| IF | 3.418 |
| 引用数 | 16 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection |