論文 - 詳細
| RRC ID | 50607 |
|---|---|
| 著者 | Mizuno K, Mizuno M, Yamauchi M, Takemura AJ, Medrano Romero V, Morikawa K. |
| タイトル | Adjacent-possible ecological niche: growth of Lactobacillus species co-cultured with Escherichia coli in a synthetic minimal medium. |
| ジャーナル | Sci Rep |
| Abstract |
In certain conditions, members of the Lactobacillus genus are auxotrophs that have fastidious requirements for growth. Notably, Lactobacillus cannot grow in M9 medium, a minimal synthetic medium used for Escherichia coli. However, we found that some Lactobacillus strains can be grown in M9 when co-cultured with E. coli K-12. In the co-culture, L. casei proliferates exponentially, reaching cell densities of 108 CFU (colony-forming unit) ml-1 in 6 h and dominating E. coli in the late growth phase. Spent medium from E. coli grown overnight lacked this growth-promoting effect on L. casei. Similarly, the effect was not observed when the species were separated by a 0.4-µm membrane. Microscopic observations showed that L. casei are embedded in the micro-scale clusters of E. coli in the early growth phase. This study describes for the first time the ability of a Lactobacillus species to grow in minimal medium when in close proximity with co-cultured bacteria. |
| 巻・号 | 7(1) |
| ページ | 12880 |
| 公開日 | 2017-10-16 |
| DOI | 10.1038/s41598-017-12894-3 |
| PII | 10.1038/s41598-017-12894-3 |
| PMID | 29038545 |
| PMC | PMC5643319 |
| MeSH | Coculture Techniques Culture Media / pharmacology* Ecosystem* Escherichia coli / cytology* Escherichia coli / drug effects Escherichia coli / growth & development Lacticaseibacillus casei / drug effects Lacticaseibacillus casei / growth & development* Staphylococcus aureus / drug effects |
| IF | 3.998 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW4277-KC JW3596-KC |