| RRC ID |
50607
|
| 著者 |
Mizuno K, Mizuno M, Yamauchi M, Takemura AJ, Medrano Romero V, Morikawa K.
|
| タイトル |
Adjacent-possible ecological niche: growth of Lactobacillus species co-cultured with Escherichia coli in a synthetic minimal medium.
|
| ジャーナル |
Sci Rep
|
| Abstract |
In certain conditions, members of the Lactobacillus genus are auxotrophs that have fastidious requirements for growth. Notably, Lactobacillus cannot grow in M9 medium, a minimal synthetic medium used for Escherichia coli. However, we found that some Lactobacillus strains can be grown in M9 when co-cultured with E. coli K-12. In the co-culture, L. casei proliferates exponentially, reaching cell densities of 108 CFU (colony-forming unit) ml-1 in 6 h and dominating E. coli in the late growth phase. Spent medium from E. coli grown overnight lacked this growth-promoting effect on L. casei. Similarly, the effect was not observed when the species were separated by a 0.4-µm membrane. Microscopic observations showed that L. casei are embedded in the micro-scale clusters of E. coli in the early growth phase. This study describes for the first time the ability of a Lactobacillus species to grow in minimal medium when in close proximity with co-cultured bacteria.
|
| 巻・号 |
7(1)
|
| ページ |
12880
|
| 公開日 |
2017-10-16
|
| DOI |
10.1038/s41598-017-12894-3
|
| PII |
10.1038/s41598-017-12894-3
|
| PMID |
29038545
|
| PMC |
PMC5643319
|
| MeSH |
Coculture Techniques
Culture Media / pharmacology*
Ecosystem*
Escherichia coli / cytology*
Escherichia coli / drug effects
Escherichia coli / growth & development
Lacticaseibacillus casei / drug effects
Lacticaseibacillus casei / growth & development*
Staphylococcus aureus / drug effects
|
| IF |
3.998
|
| 引用数 |
0
|
| リソース情報 |
| 原核生物(大腸菌) |
JW4277-KC
JW3596-KC |
