RRC ID 5063
Author Lee FL, Kuo HP, Tai CJ, Yokota A, Lo CC.
Title Paenibacillus taiwanensis sp. nov., isolated from soil in Taiwan.
Journal Int. J. Syst. Evol. Microbiol.
Abstract Among a large collection of Taiwanese soil isolates, a novel Gram-variable, rod-shaped, motile and endospore-forming bacterial strain, designated G-soil-2-3(T), was isolated from farmland soil in Wu-Feng, Taiwan. The isolate was subjected to a polyphasic study including 16S rRNA gene sequence analysis, DNA-DNA hybridization experiments, fatty acid analysis and comparative phenotypic characterization. 16S rRNA gene sequence analysis indicated that the organism belongs within the genus Paenibacillus. It contained menaquinone MK-7 as the predominant isoprenoid quinone and anteiso-C(15 : 0) (40.5 %), iso-C(15 : 0) (13.1 %), iso-C(16 : 0) (10.8 %) and anteiso-C(17 : 0) (7.3 %) as the major fatty acids. Phylogenetically, the closest relatives of strain G-soil-2-3(T) were the type strains of Paenibacillus assamensis, Paenibacillus alvei and Paenibacillus apiarius, with 16S rRNA gene sequence similarity of 95.7, 95 and 95.2 %, respectively. DNA-DNA hybridization experiments showed levels of relatedness of 2.8-9.0 % of strain G-soil-2-3(T) with these strains. The G+C content of the DNA was 44.6 mol%. Strain G-soil-2-3(T) was clearly distinguishable from P. assamensis, P. alvei and P. apiarius and thus represents a novel species of the genus Paenibacillus, for which the name Paenibacillus taiwanensis sp. nov. is proposed. The type strain is G-soil-2-3(T) (=BCRC 17411(T)=IAM 15414(T)=LMG 23799(T)=DSM 18679(T)).
Volume 57(Pt 6)
Pages 1351-1354
Published 2007-6
DOI 10.1099/ijs.0.64764-0
PMID 17551057
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Genes, rRNA Gram-Positive Endospore-Forming Rods / chemistry Gram-Positive Endospore-Forming Rods / classification* Gram-Positive Endospore-Forming Rods / isolation & purification* Gram-Positive Endospore-Forming Rods / physiology Locomotion Molecular Sequence Data Nucleic Acid Hybridization Phylogeny Quinones / analysis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Soil Microbiology* Taiwan
IF 2.166
Times Cited 11
WOS Category MICROBIOLOGY
Resource
General Microbes JCM13186