RRC ID 50649
著者 Yoshida W, Saikyo H, Nakabayashi K, Yoshioka H, Bay DH, Iida K, Kawai T, Hata K, Ikebukuro K, Nagasawa K, Karube I.
タイトル Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand.
ジャーナル Sci Rep
Abstract G-quadruplex (G4) is a DNA secondary structure that has been found to play regulatory roles in the genome. The identification of G4-forming sequences is important to study the specific structure-function relationships of such regions. In the present study, we developed a method for identification of G4 clusters on genomic DNA by high-throughput sequencing of genomic DNA amplified via whole-genome amplification (WGA) in the presence of a G4 ligand. The G4 ligand specifically bound to G4 structures on genomic DNA; thus, DNA polymerase was arrested on the G4 structures stabilised by G4 ligand. We utilised the telomestatin derivative L1H1-7OTD as a G4 ligand and demonstrated that the efficiency of amplification of the G4 cluster regions was lower than that of the non-G4-forming regions. By high-throughput sequencing of the WGA products, 9,651 G4 clusters were identified on human genomic DNA. Among these clusters, 3,766 G4 clusters contained at least one transcriptional start site, suggesting that genes are regulated by G4 clusters rather than by one G4 structure.
巻・号 8(1)
ページ 3116
公開日 2018-2-15
DOI 10.1038/s41598-018-21514-7
PII 10.1038/s41598-018-21514-7
PMID 29449667
PMC PMC5814564
MeSH DNA / analysis DNA / genetics DNA-Directed DNA Polymerase / metabolism G-Quadruplexes* Genome, Human Genomic Instability High-Throughput Nucleotide Sequencing / methods* Humans Ligands Nucleic Acid Synthesis Inhibitors / pharmacology Oxazoles / pharmacology* Transcription, Genetic
IF 3.998
引用数 11
リソース情報
ヒト・動物細胞 HeLa(RCB0007)