RRC ID 50654
Author Doke M, Fukamachi H, Morisaki H, Arimoto T, Kataoka H, Kuwata H.
Title Nucleases from Prevotella intermedia can degrade neutrophil extracellular traps.
Journal Mol Oral Microbiol
Abstract Periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. These bacteria are able to colonize the periodontal region by evading the host immune response. Neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (NETs). These are extracellular net-like fibers comprising DNA and antimicrobial components such as histones, LL-37, defensins, myeloperoxidase, and neutrophil elastase from neutrophils that disarm and kill bacteria extracellularly. Bacterial nuclease degrades the NETs to escape NET killing. It has now been shown that extracellular nucleases enable bacteria to evade this host antimicrobial mechanism, leading to increased pathogenicity. Here, we compared the DNA degradation activity of major Gram-negative periodontopathogenic bacteria, Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. We found that Pr. intermedia showed the highest DNA degradation activity. A genome search of Pr. intermedia revealed the presence of two genes, nucA and nucD, putatively encoding secreted nucleases, although their enzymatic and biological activities are unknown. We cloned nucA- and nucD-encoding nucleases from Pr. intermedia ATCC 25611 and characterized their gene products. Recombinant NucA and NucD digested DNA and RNA, which required both Mg2+ and Ca2+ for optimal activity. In addition, NucA and NucD were able to degrade the DNA matrix comprising NETs.
Volume 32(4)
Pages 288-300
Published 2017-8-1
DOI 10.1111/omi.12171
PMID 27476978
PMC PMC5516193
MeSH Bacteria / enzymology Bacteria / genetics Bacteria / pathogenicity Bacterial Proteins / genetics Bacterial Proteins / metabolism Culture Media / chemistry DNA / metabolism* Deoxyribonucleases / metabolism* Extracellular Traps / metabolism* Fusobacterium nucleatum / enzymology Fusobacterium nucleatum / genetics Fusobacterium nucleatum / pathogenicity Genome, Bacterial Humans Neutrophils / immunology* Neutrophils / microbiology Porphyromonas gingivalis / enzymology Porphyromonas gingivalis / genetics Porphyromonas gingivalis / pathogenicity Prevotella intermedia / enzymology* Prevotella intermedia / genetics Prevotella intermedia / pathogenicity RNA / metabolism
IF 2.905
Times Cited 15
General Microbes JCM 12248