RRC ID 50665
Author Li Y, Gu Z, Zhang L, Ding Z, Shi G.
Title Inducible expression of trehalose synthase in Bacillus licheniformis.
Journal Protein Expr Purif
Abstract Trehalose synthase (TreS) could transform maltose into trehalose via isomerization. It is a crucial enzyme in the process of trehalose enzymatical transformation. In this study, plasmid-based inducible expression systems were constructed to produce Thermomonospora curvata TreS in B. licheniformis. Xylose operons from B. subtilis, B. licheniformis and B. megaterium were introduced to regulate the expression of the gene encoding TreS. It was functionally expressed, and the BlsTs construct yielded the highest enzyme activity (12.1 U/mL). Furthermore, the effect of different cultural conditions on the inducible expression of BlsTs was investigated, and the optimal condition was as follows: 4% maltodextrin, 0.4% soybean powder, 1% xylose added after 10 h of growth and an induction time of 12 h at 37 °C. As a result, the maximal yield reached 24.7 U/mL. This study contributes to the industrial application of B. licheniformis, a GRAS workhorse for enzyme production.
Volume 130
Pages 115-122
Published 2017-2
DOI 10.1016/j.pep.2016.10.005
PII S1046-5928(16)30218-2
PMID 27751933
MeSH Actinobacteria / enzymology Actinobacteria / genetics* Bacillus licheniformis / genetics Bacillus licheniformis / metabolism* Bacterial Proteins* / biosynthesis Bacterial Proteins* / genetics Bacterial Proteins* / isolation & purification Gene Expression* Glucosyltransferases* / biosynthesis Glucosyltransferases* / genetics Glucosyltransferases* / isolation & purification Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / isolation & purification
IF 1.291
Times Cited 4
Resource
General Microbes JCM 3096