| RRC ID |
50672
|
| Author |
Dash HR, Basu S, Das S.
|
| Title |
Evidence of mercury trapping in biofilm-EPS and mer operon-based volatilization of inorganic mercury in a marine bacterium Bacillus cereus BW-201B.
|
| Journal |
Arch Microbiol
|
| Abstract |
Biofilm-forming mercury-resistant marine bacterium Bacillus cereus BW-201B has been explored to evident that the bacterial biofilm-EPS (exopolymers) trap inorganic mercury but subsequently release EPS-bound mercury for induction of mer operon-mediated volatilization of inorganic mercury. The isolate was able to tolerate 50 ppm of mercury and forms biofilm in presence of mercury. mer operon-mediated volatilization was confirmed, and -SH was found to be the key functional group of bacterial EPS responsible for mercury binding. Biofilm-EPS-bound mercury was found to be internalized to the bacterial system as confirmed by reversible conformational change of -SH group and increased expression level of merA gene in a timescale experiment. Biofilm-EPS trapped Hg after 24 h of incubation, and by 96 h, the volatilization process reaches to its optimum confirming the internalization of EPS-bound mercury to the bacterial cells. Biofilm disintegration at the same time corroborates the results.
|
| Volume |
199(3)
|
| Pages |
445-455
|
| Published |
2017-4-1
|
| DOI |
10.1007/s00203-016-1317-2
|
| PII |
10.1007/s00203-016-1317-2
|
| PMID |
27815566
|
| MeSH |
Bacillus cereus / genetics*
Bacillus cereus / metabolism*
Biofilms*
Mercury / metabolism*
Operon / genetics*
Oxidoreductases / genetics*
Oxidoreductases / metabolism
Volatilization
|
| IF |
1.884
|
| Times Cited |
2
|
| Resource |
| General Microbes |
JCM 30391 |
