RRC ID 50672
Author Dash HR, Basu S, Das S.
Title Evidence of mercury trapping in biofilm-EPS and mer operon-based volatilization of inorganic mercury in a marine bacterium Bacillus cereus BW-201B.
Journal Arch. Microbiol.
Abstract Biofilm-forming mercury-resistant marine bacterium Bacillus cereus BW-201B has been explored to evident that the bacterial biofilm-EPS (exopolymers) trap inorganic mercury but subsequently release EPS-bound mercury for induction of mer operon-mediated volatilization of inorganic mercury. The isolate was able to tolerate 50 ppm of mercury and forms biofilm in presence of mercury. mer operon-mediated volatilization was confirmed, and -SH was found to be the key functional group of bacterial EPS responsible for mercury binding. Biofilm-EPS-bound mercury was found to be internalized to the bacterial system as confirmed by reversible conformational change of -SH group and increased expression level of merA gene in a timescale experiment. Biofilm-EPS trapped Hg after 24 h of incubation, and by 96 h, the volatilization process reaches to its optimum confirming the internalization of EPS-bound mercury to the bacterial cells. Biofilm disintegration at the same time corroborates the results.
Volume 199(3)
Pages 445-455
Published 2017-4
DOI 10.1007/s00203-016-1317-2
PII 10.1007/s00203-016-1317-2
PMID 27815566
MeSH Bacillus cereus / genetics* Bacillus cereus / metabolism* Biofilms* Mercury / metabolism* Operon / genetics* Oxidoreductases / genetics* Oxidoreductases / metabolism Volatilization
IF 1.642
General Microbes JCM 30391