| RRC ID |
5068
|
| Author |
Gutiérrez MC, Castillo AM, Kamekura M, Xue Y, Ma Y, Cowan DA, Jones BE, Grant WD, Ventosa A.
|
| Title |
Halopiger xanaduensis gen. nov., sp. nov., an extremely halophilic archaeon isolated from saline Lake Shangmatala in Inner Mongolia, China.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Strain SH-6(T) was isolated from the sediment of Lake Shangmatala, a saline lake in Inner Mongolia (China). Cells were pleomorphic. The organism was neutrophilic and required at least 2.5 M (15 %) NaCl, but not MgCl(2), for growth; optimal growth occurred at 4.3 M (25 %) NaCl. The G+C content of its DNA was 63.1 mol%. 16S rRNA gene sequence analysis revealed that strain SH-6(T) is a member of the family Halobacteriaceae, but there was a low level of similarity with other members of this family. Highest sequence similarity (94.6 %) was obtained with the 16S rRNA genes of the type strains of Natronolimnobius innermongolicus and Natronolimnobius baerhuensis. Polar lipid analyses revealed that strain SH-6(T) contains phosphatidylglycerol and phosphatidylglyceromethylphosphate, derived from both C(20)C(20) and C(20)C(25) glycerol diethers together with the glycolipid S(2)-DGD-1. On the basis of the data obtained, the new isolate could not be classified in any recognized genus. Strain SH-6(T) is thus considered to represent a novel species in a new genus within the family Halobacteriaceae, order Halobacteriales, for which the name Halopiger xanaduensis gen. nov., sp. nov. is proposed. The type strain of Halopiger xanaduensis is SH-6(T) (=CECT 7173(T)=CGMCC 1.6379(T)=JCM 14033(T)).
|
| Volume |
57(Pt 7)
|
| Pages |
1402-1407
|
| Published |
2007-7-1
|
| DOI |
10.1099/ijs.0.65001-0
|
| PMID |
17625165
|
| MeSH |
Base Composition
China
DNA, Archaeal / chemistry
DNA, Archaeal / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Genes, rRNA
Geologic Sediments / microbiology*
Halobacteriaceae / chemistry
Halobacteriaceae / classification*
Halobacteriaceae / isolation & purification*
Halobacteriaceae / physiology
Magnesium Chloride / metabolism
Microscopy, Phase-Contrast
Molecular Sequence Data
Phospholipids / analysis
Phylogeny
RNA, Archaeal / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Sodium Chloride / metabolism
|
| IF |
2.415
|
| Times Cited |
38
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM14033 |
