RRC ID 5069
Author Plumb JJ, Haddad CM, Gibson JA, Franzmann PD.
Title Acidianus sulfidivorans sp. nov., an extremely acidophilic, thermophilic archaeon isolated from a solfatara on Lihir Island, Papua New Guinea, and emendation of the genus description.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A novel, extremely thermoacidophilic, obligately chemolithotrophic archaeon (strain JP7(T)) was isolated from a solfatara on Lihir Island, Papua New Guinea. Cells of this organism were non-motile, Gram-negative staining, irregular-shaped cocci, 0.5-1.5 microm in size, that grew aerobically by oxidation of sulfur, Fe(2+) or mineral sulfides. Cells grew anaerobically using Fe(3+) as a terminal electron acceptor and H(2)S as an electron donor but did not oxidize hydrogen with elemental sulfur as electron acceptor. Strain JP7(T) grew optimally at 74 degrees C (temperature range 45-83 degrees C) and pH 0.8-1.4 (pH range 0.35-3.0). On the basis of 16S rRNA gene sequence similarity, strain JP7(T) was shown to belong to the Sulfolobaceae, being most closely related to the type strains of Acidianus ambivalens (93.7 %) and Acidianus infernus (93.6 %). Cell-membrane lipid structure, DNA base composition and 16S rRNA gene sequence similarity data support the placement of this strain in the genus Acidianus. Differences in aerobic and anaerobic metabolism, temperature and pH range for growth, and 16S rRNA gene sequence differentiate strain JP7(T) from recognized species of the genus Acidianus, and an emendation of the description of the genus is proposed. Strain JP7(T) is considered to represent a novel species of the genus Acidianus, for which the name Acidianus sulfidivorans sp. nov. is proposed. The type strain is JP7(T) (=DSM 18786(T)=JCM 13667(T)).
Volume 57(Pt 7)
Pages 1418-23
Published 2007-7
DOI 10.1099/ijs.0.64846-0
PII 57/7/1418
PMID 17625168
MeSH Acidianus / classification* Acidianus / genetics Acidianus / isolation & purification* Acidianus / metabolism Aerobiosis Base Composition Cell Membrane / chemistry DNA, Archaeal / chemistry DNA, Archaeal / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Ferric Compounds / metabolism Ferrous Compounds / metabolism Genes, rRNA Hydrogen / metabolism Hydrogen-Ion Concentration Lipids / analysis Locomotion Molecular Sequence Data Oxidation-Reduction Papua New Guinea Phylogeny RNA, Archaeal / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Soil Microbiology* Sulfides / metabolism Sulfur / metabolism Temperature
IF 2.166
Times Cited 31
General Microbes JCM13667