| RRC ID |
50731
|
| Author |
Ben Hania W, Joseph M, Bunk B, Spröer C, Klenk HP, Fardeau ML, Spring S.
|
| Title |
Characterization of the first cultured representative of a Bacteroidetes clade specialized on the scavenging of cyanobacteria.
|
| Journal |
Environ Microbiol
|
| Abstract |
The anaerobic, mesophilic and moderately halophilic strain L21-Spi-D4T was recently isolated from the suboxic zone of a hypersaline cyanobacterial mat using protein-rich extracts of Arthrospira (formerly Spirulina) platensis as substrate. Phylogenetic analyses based on 16S rRNA genes indicated an affiliation of the novel strain with the Bacteroidetes clade MgMjR-022, which is widely distributed and abundant in hypersaline microbial mats and heretofore comprised only sequences of uncultured bacteria. Analyses of the complete genome sequence of strain L21-Spi-D4T revealed a possible specialization on the degradation of cyanobacterial biomass. Besides genes for enzymes degrading specific cyanobacterial proteins a conspicuous transport complex for the polypeptide cyanophycin could be identified that is homologous to typical polysaccharide utilization loci of Bacteroidetes. A distinct and reproducible co-occurrence pattern of environmental 16S rRNA gene sequences of the MgMjR-022 clade and cyanobacteria in the suboxic zone of hypersaline mats points to a specific dependence of members of this clade on decaying cyanobacteria. Based on a comparative analysis of phenotypic, genomic and ecological characteristics we propose to establish the novel taxa Salinivirga cyanobacteriivorans gen. nov., sp. nov., represented by the type strain L21-Spi-D4T , and Salinivirgaceae fam. nov., comprising sequences of the MgMjR-022 clade.
|
| Volume |
19(3)
|
| Pages |
1134-1148
|
| Published |
2017-3-1
|
| DOI |
10.1111/1462-2920.13639
|
| PMID |
27943642
|
| MeSH |
Animals
Bacteroidetes / classification
Bacteroidetes / genetics
Bacteroidetes / growth & development
Bacteroidetes / isolation & purification*
Base Composition
Cyanobacteria / genetics
Cyanobacteria / metabolism
Fatty Acids / metabolism
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sodium Chloride / metabolism
|
| IF |
4.933
|
| Times Cited |
12
|
| Resource |
| General Microbes |
JCM 31231 |
