Reference - Detail
| RRC ID | 50769 |
|---|---|
| Author | Kono T, Mehrotra S, Endo C, Kizu N, Matusda M, Kimura H, Mizohata E, Inoue T, Hasunuma T, Yokota A, Matsumura H, Ashida H. |
| Title | A RuBisCO-mediated carbon metabolic pathway in methanogenic archaea. |
| Journal | Nat Commun |
| Abstract |
Two enzymes are considered to be unique to the photosynthetic Calvin-Benson cycle: ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for CO2 fixation, and phosphoribulokinase (PRK). Some archaea possess bona fide RuBisCOs, despite not being photosynthetic organisms, but are thought to lack PRK. Here we demonstrate the existence in methanogenic archaea of a carbon metabolic pathway involving RuBisCO and PRK, which we term 'reductive hexulose-phosphate' (RHP) pathway. These archaea possess both RuBisCO and a catalytically active PRK whose crystal structure resembles that of photosynthetic bacterial PRK. Capillary electrophoresis-mass spectrometric analysis of metabolites reveals that the RHP pathway, which differs from the Calvin-Benson cycle only in a few steps, is active in vivo. Our work highlights evolutionary and functional links between RuBisCO-mediated carbon metabolic pathways in methanogenic archaea and photosynthetic organisms. Whether the RHP pathway allows for autotrophy (that is, growth exclusively with CO2 as carbon source) remains unknown. |
| Volume | 8 |
| Pages | 14007 |
| Published | 2017-1-13 |
| DOI | 10.1038/ncomms14007 |
| PII | ncomms14007 |
| PMID | 28082747 |
| PMC | PMC5241800 |
| MeSH | Archaeal Proteins / chemistry Archaeal Proteins / genetics Archaeal Proteins / metabolism* Carbon / metabolism Euryarchaeota / classification Euryarchaeota / enzymology* Euryarchaeota / genetics Euryarchaeota / metabolism Metabolic Networks and Pathways Phosphotransferases (Alcohol Group Acceptor) / chemistry Phosphotransferases (Alcohol Group Acceptor) / genetics Phosphotransferases (Alcohol Group Acceptor) / metabolism Photosynthesis Phylogeny Ribulose-Bisphosphate Carboxylase / chemistry Ribulose-Bisphosphate Carboxylase / genetics Ribulose-Bisphosphate Carboxylase / metabolism* |
| IF | 12.121 |
| Times Cited | 39 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 63 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 10133 |