| RRC ID |
50795
|
| Author |
Zachariah S, Kumari P, Das SK.
|
| Title |
Sulfitobacter pontiacus subsp. fungiae subsp. nov., Isolated from Coral Fungia seychellensis from Andaman Sea, and Description of Sulfitobacter pontiacus subsp. pontiacus subsp. nov.
|
| Journal |
Curr Microbiol
|
| Abstract |
Two closely related aerobic, Gram reaction-negative rod-shaped bacteria (S7-75T and S7-80) were isolated from mucus of coral Fungia seychellensis from Andaman Sea, India. Heterotrophic growth on marine agar was observed at 4-35 °C and pH 6.5-10.5; optimum growth occurred at 25-30 °C and pH 7-8. 16 S rRNA sequence analysis confirmed the strains belonged to the genus Sulfitobacter and the two isolates shared more than 99.28% pairwise sequence similarity. DNA-DNA similarity between two isolates S7-75T and S7-80 was above 96%. Strain S7-75T showed maximum 16S rRNA similarity of 99.64% with Sulfitobacter pontiacus LMG 19752T. However, DNA-DNA relatedness between strain S7-75T and S. pontiacus LMG 19752T confirmed the placement of strain S7-75T as subspecies under the species S. pontiacus. Further, pulsed-field gel electrophoresis (PFGE), REP-PCR, ERIC-PCR fingerprint patterns and lipid profiles also differentiated strain S7-75T from the reference strain of S. pontiacus LMG 19752T. The DNA G+C content was 59.8 mol%. Q10 was the major respiratory quinone. Based on polyphasic analysis, the isolate S7-75T represents a subspecies of S. pontiacus for which the name S. pontiacus subsp. fungiae subsp. nov. is proposed with S7-75T (=JCM 31094T = LMG 29158T) as type strain.
|
| Volume |
74(3)
|
| Pages |
404-412
|
| Published |
2017-3-1
|
| DOI |
10.1007/s00284-017-1200-7
|
| PII |
10.1007/s00284-017-1200-7
|
| PMID |
28184991
|
| MeSH |
Animals
Anthozoa / microbiology*
Base Composition / genetics
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Gram-Negative Aerobic Bacteria / classification
Gram-Negative Aerobic Bacteria / genetics*
Rhodobacteraceae / classification
Rhodobacteraceae / genetics
|
| IF |
1.746
|
| Times Cited |
2
|
| Resource |
| General Microbes |
JCM 31094 |
