RRC ID 50813
Author Lee JJ, Park SJ, Lee YH, Lee SY, Park S, Cho YJ, Kim MK, Ten LN, Jung HY.
Title Spirosoma luteolum sp. nov. isolated from water.
Journal J. Microbiol.
Abstract A novel Gram-negative and rod-shaped bacterial strain, designated as 16F6ET, was isolated from a water sample. Cells were yellowish in color and catalase- and oxidase-positive. The strain grew at 10-37°C (optimum at 25°C) but not at 4 and 42°C, and pH 5-7 (optimum at pH 7). It showed moderate resistance to gamma-ray irradiation. Comparative phylogenetic analysis showed that strain 16F6ET belonged to the family Cytophagaceae of the class Cytophagia. Furthermore, this isolate showed relatively low 16S rRNA gene sequence similarities (90.7-93.1%) to the members of the genus Spirosoma. The major fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:1 ω5c, C16:0 N alcohol, and C16:0. The polar lipid profile indicated presence of phosphatidylethanolamine, unknown aminophospholipids, an unknown amino lipid, unknown phospholipids, and unknown polar lipids. The predominant isoprenoid quinone was MK-7. The genomic DNA G+C content of strain 16F6ET was 56.5 mol%. Phenotypic, phylogenetic, and chemotaxonomic properties indicated that isolate 16F6ET represents a novel species within the genus Spirosoma, for which the name Spirosoma luteolum sp. nov. is proposed. The type strain is 16F6ET (=KCTC 52199T =JCM 31411T).
Volume 55(4)
Pages 247-252
Published 2017-4
DOI 10.1007/s12275-017-6455-y
PII 10.1007/s12275-017-6455-y
PMID 28251547
MeSH Base Composition Catalase / analysis Cluster Analysis Cytophagaceae / classification* Cytophagaceae / genetics Cytophagaceae / isolation & purification* Cytophagaceae / physiology Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Gamma Rays Hydrogen-Ion Concentration Oxidoreductases / analysis Phospholipids / analysis Phylogeny Pigments, Biological / metabolism Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Temperature Water Microbiology*
IF 2.319
Resource
General Microbes JCM 31411