論文 - 詳細
| RRC ID | 50821 |
|---|---|
| 著者 | Kitagawa K, Oda T, Saito H, Araki A, Gonoi R, Shigemura K, Hashii Y, Katayama T, Fujisawa M, Shirakawa T. |
| タイトル | Development of oral cancer vaccine using recombinant Bifidobacterium displaying Wilms' tumor 1 protein. |
| ジャーナル | Cancer Immunol Immunother |
| Abstract |
Several types of vaccine-delivering tumor-associated antigens (TAAs) have been developed in basic and clinical research. Wilms' tumor 1 (WT1), identified as a gene responsible for pediatric renal neoplasm, is one of the most promising TAA for cancer immunotherapy. Peptide and dendritic cell-based WT1 cancer vaccines showed some therapeutic efficacy in clinical and pre-clinical studies but as yet no oral WT1 vaccine can be administrated in a simple and easy way. In the present study, we constructed a novel oral cancer vaccine using a recombinant Bifidobacterium longum displaying WT1 protein. B. longum 420 was orally administered into mice inoculated with WT1-expressing tumor cells for 4 weeks to examine anti-tumor effects. To analyze the WT1-specific cellular immune responses to oral B. longum 420, mice splenocytes were isolated and cytokine production and cytotoxic activities were determined. Oral administrations of B. longum 420 significantly inhibited WT1-expressing tumor growth and prolonged survival in mice. Immunohistochemical study and immunological assays revealed that B. longum 420 substantially induced tumor infiltration of CD4+T and CD8+T cells, systemic WT1-specific cytokine production, and cytotoxic activity mediated by WT1-epitope specific cytotoxic T lymphocytes, with no apparent adverse effects. Our novel oral cancer vaccine safely induced WT1-specific cellular immunity via activation of the gut mucosal immune system and achieved therapeutic efficacy with several practical advantages over existing non-oral vaccines. |
| 巻・号 | 66(6) |
| ページ | 787-798 |
| 公開日 | 2017-6-1 |
| DOI | 10.1007/s00262-017-1984-0 |
| PII | 10.1007/s00262-017-1984-0 |
| PMID | 28299466 |
| PMC | PMC11028424 |
| MeSH | Animals Bifidobacterium / immunology* Cancer Vaccines / administration & dosage* Cancer Vaccines / immunology Female Immunotherapy* Mice Mice, Inbred C57BL Mouth Neoplasms / immunology* Mouth Neoplasms / metabolism Mouth Neoplasms / mortality Mouth Neoplasms / therapy* Survival Rate T-Lymphocytes, Cytotoxic / immunology WT1 Proteins / immunology* |
| IF | 5.442 |
| 引用数 | 9 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 31944 |