RRC ID 50867
Author Sajadi E, Babaipour V, Deldar AA, Yakhchali B, Fatemi SS.
Title Enhancement of crystallinity of cellulose produced by Escherichia coli through heterologous expression of bcsD gene from Gluconacetobacter xylinus.
Journal Biotechnol. Lett.
Abstract OBJECTIVES:To evaluate the crystallinity index of the cellulose produced by Escherichia coli Nissle 1917 after heterologous expression of the cellulose synthase subunit D (bcsD) gene of Gluconacetobacter xylinus BPR2001.
RESULTS:The bcsD gene of G. xylinus BPR2001 was expressed in E. coli and its protein product was visualized using SDS-PAGE. FTIR analysis showed that the crystallinity index of the cellulose produced by the recombinants was 0.84, which is 17% more than that of the wild type strain. The increased crystallinity index was also confirmed by X-ray diffraction analysis. The cellulose content was not changed significantly after over-expressing the bcsD.
CONCLUSION:The bcsD gene can improve the crystalline structure of the bacterial cellulose but there is not any significant difference between the amounts of cellulose produced by the recombinant and wild type E. coli Nissle 1917.
Volume 39(9)
Pages 1395-1401
Published 2017-9
DOI 10.1007/s10529-017-2366-6
PII 10.1007/s10529-017-2366-6
PMID 28573539
MeSH Cellulose / chemistry Cellulose / metabolism* Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Gene Expression Gluconacetobacter xylinus / enzymology* Gluconacetobacter xylinus / genetics Glucosyltransferases / genetics Glucosyltransferases / metabolism* Metabolic Engineering / methods* Protein Subunits / genetics Protein Subunits / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism* Spectroscopy, Fourier Transform Infrared X-Ray Diffraction
IF 1.846
Resource
General Microbes JCM 9730