RRC ID |
50903
|
著者 |
Nakayama T, Yamazaki T, Yo A, Tone K, Mahdi Alshahni M, Fujisaki R, Makimura K.
|
タイトル |
Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method.
|
ジャーナル |
Biocontrol Sci
|
Abstract |
Loop-mediated isothermal amplification (LAMP) is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans (Ascomycota), Cryptococcus neoformans (Basidiomycota), and Mucor racemosus (Mucorales). The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction. In this study, we evaluated the correlation between colony forming units (CFU) and LAMP detection rate using the LAMP method for environmental fungi. The LAMP method should be a useful means of detecting fungi in indoor environments, disaster areas, or even in confined manned spacecraft to prevent allergies or infections caused by fungi.
|
巻・号 |
22(2)
|
ページ |
97-104
|
公開日 |
2017-1-1
|
DOI |
10.4265/bio.22.97
|
PMID |
28659561
|
MeSH |
Basidiomycota / genetics
Basidiomycota / isolation & purification
Candida / genetics
Candida / isolation & purification
Cryptococcus / genetics
Cryptococcus / isolation & purification
DNA Primers / genetics*
Food Microbiology / methods*
Fungal Proteins / genetics*
Nucleic Acid Amplification Techniques / methods*
Sensitivity and Specificity
|
IF |
0.833
|
引用数 |
5
|
リソース情報 |
一般微生物 |
JCM 2061 |