論文 - 詳細
| RRC ID | 50915 |
|---|---|
| 著者 | Nguyen TM, Kim J. |
| タイトル | A rapid and simple method for identifying bacterial polar lipid components in wet biomass. |
| ジャーナル | J Microbiol |
| Abstract |
There are marked differences between wet and freeze-dried cells with regard to the identification of polar lipid components. The determination of the polar lipid composition of freeze-dried cells is well established. However, several approaches to identifying polar lipid components in wet cells have met with limited success owing to the presence of non-polar compounds in the extracts, resulting in a lipid composition with a narrow scope. In this study, we surveyed the lipid profiles of the wet biomasses of three Gram-positive (Microbacterium lacticum, Rhodococcus koreensis, and Streptomyces longwoodensis) and two Gram-negative (Pseudomonas aeruginosa and Novosphingobium capsulatum) bacteria; the results were comparable in quality to those obtained using a standard freeze-dried approach. Moreover, our improved method ensures simple lipid extraction. Overall, the results of the analysis showed minor lipid profile differences between the two approaches with regard to quantity, and lipid identification was consistent in both methods for all species. |
| 巻・号 | 55(8) |
| ページ | 635-639 |
| 公開日 | 2017-8-1 |
| DOI | 10.1007/s12275-017-7092-1 |
| PII | 10.1007/s12275-017-7092-1 |
| PMID | 28674971 |
| MeSH | Gram-Negative Bacteria / chemistry* Gram-Positive Bacteria / chemistry* Phospholipids / analysis* Phospholipids / isolation & purification* |
| IF | 2.845 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 10743 |