論文 - 詳細
| RRC ID | 5093 |
|---|---|
| 著者 | Yang SH, Lee JH, Ryu JS, Kato C, Kim SJ. |
| タイトル | Shewanella donghaensis sp. nov., a psychrophilic, piezosensitive bacterium producing high levels of polyunsaturated fatty acid, isolated from deep-sea sediments. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A Gram-negative, motile, rod-shaped, psychrophilic bacterium, LT17T, was isolated from deep-sea sediments (3300 m depth) of the East Sea (Sea of Japan). Optimal growth of LT17T requires the presence of 2.5 % (w/v) NaCl, a pH of 7.0-7.5 and a temperature of 17 degrees C. The isolate grows optimally under a hydrostatic pressure of 10 MPa and growth is possible between 0.1 and <30 MPa. The novel strain is positive in tests for catalase, oxidase, lipase, beta-glucosidase and gelatinase activities and reduces nitrate to nitrate. The predominant cellular fatty acids are iso-C13 : 0, iso-C15 : 0, C16 : 0, C16 : 1omega7 and C20 : 5omega3. The DNA G+C content of strain LT17T is 38.8 mol%. Phylogenetic analysis of 16S rRNA gene sequences places this bacterium in the class Gammaproteobacteria, within the genus Shewanella. The closest relatives of strain LT17T are Shewanella japonica (97.8 % gene sequence similarity), Shewanella pacifica (97.5 %), Shewanella olleyana (96.8 %), Shewanella frigidimarina (96.5 %) and Shewanella gelidimarina (95.4 %). The DNA-DNA hybridization levels between the novel isolate and its closest known phylogenetic relatives, S. japonica and S. pacifica, are lower than 14 %. On the basis of this polyphasic evidence, strain LT17T represents a novel species of the genus Shewanella, for which the name Shewanella donghaensis sp. nov. is proposed. The type strain is LT17T (=KCTC 10635BPT=JCM 12524T). |
| 巻・号 | 57(Pt 2) |
| ページ | 208-212 |
| 公開日 | 2007-2-1 |
| DOI | 10.1099/ijs.0.64469-0 |
| PMID | 17267951 |
| MeSH | Bacterial Proteins / analysis Bacterial Typing Techniques Base Composition DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Enzymes / analysis Fatty Acids / analysis Fatty Acids, Unsaturated / biosynthesis* Genes, rRNA / genetics Geologic Sediments / microbiology* Hydrogen-Ion Concentration Hydrostatic Pressure Japan Molecular Sequence Data Movement Nucleic Acid Hybridization Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Seawater / microbiology* Sequence Analysis, DNA Shewanella / classification* Shewanella / cytology Shewanella / isolation & purification Shewanella / metabolism* Temperature Water Microbiology |
| IF | 2.415 |
| 引用数 | 22 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM12524 |