RRC ID 5095
Author Kim HG, Doronina NV, Trotsenko YA, Kim SW.
Title Methylophaga aminisulfidivorans sp. nov., a restricted facultatively methylotrophic marine bacterium.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A novel restricted facultatively methylotrophic marine strain, MP(T), possessing the ribulose monophosphate pathway of C(1)-carbon compound assimilation was isolated from a seawater sample obtained from Mokpo, South Korea. The novel isolate is aerobic, Gram-negative, asporogenous and a non-motile short rod. It grows well on methanol, methylated amines, dimethylsulfide and DMSO. Optimal growth occurs with 3 % NaCl at 30 degrees C and pH 7.0. Fructose is utilized as a multicarbon source. Growth factors are not required and vitamin B(12) does not stimulate growth. The cellular fatty acid profile of the novel strain consists primarily of straight-chain saturated C(16 : 0) and unsaturated C(16 : 1) acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content is 44.9 mol% (T(m)). Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (25-41 %) with the type strains of marine methylotrophs belonging to the genus Methylophaga, it is suggested that isolate MP(T) represents a novel species, Methylophaga aminisulfidivorans sp. nov. (type strain MP(T)=KCTC 12909(T)=VKM B-2441(T)=JCM 14647(T)).
Volume 57(Pt 9)
Pages 2096-2101
Published 2007-9
DOI 10.1099/ijs.0.65139-0
PMID 17766878
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Carbon / metabolism* DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Dimethyl Sulfoxide / metabolism Fatty Acids / analysis Fructose / metabolism Genes, rRNA Hydrogen-Ion Concentration Korea Methanol / metabolism Methylamines / metabolism Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Piscirickettsiaceae / classification* Piscirickettsiaceae / genetics Piscirickettsiaceae / isolation & purification* Piscirickettsiaceae / physiology RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Seawater / microbiology* Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sodium Chloride / metabolism Sulfides / metabolism Temperature Ubiquinone / analysis Vitamin B 12 / metabolism
IF 2.166
Times Cited 30
General Microbes JCM14647