Reference - Detail
| RRC ID | 50951 |
|---|---|
| Author | Li Y, Wang Y, Tu T, Zhang D, Ma R, You S, Wang X, Yao B, Luo H, Xu B. |
| Title | Two acidic, thermophilic GH28 polygalacturonases from Talaromyces leycettanus JCM 12802 with application potentials for grape juice clarification. |
| Journal | Food Chem |
| Abstract |
Efficient hydrolysis of pectic materials to sugars requires the synergistic action of endo- and exo-polygalacturonases. Two novel polygalacturonases (exo-TePG28a and endo-TePG28b) were identified in Talaromyces leycettanus JCM12802, overexpressed in Pichia pastoris, and characterized in this report. The specific activities of TePG28a and TePG28b towards polygalacturonic acid were 280±9 and 25,900±502U/mg, respectively. Both enzymes exhibited optimal activities at pH 3.5 and retained highly stable over a broad pH range of 2.0-7.0. Distinct from most fungal polygalacturonases that have low temperature optima, TePG28a and TePG28b were optimally active at 70°C. When treated the grape juice with the enzyme combination (the unit ratio of TePG28a:TePG28b was 1:4), higher pectin-degrading efficiency (up to 140%) was achieved, and light transmittance was improved from 14% to 82%. These favorable enzymatic properties make TePG28a and TePG28b attractive for the applications in the juice industry. |
| Volume | 237 |
| Pages | 997-1003 |
| Published | 2017-12-15 |
| DOI | 10.1016/j.foodchem.2017.06.037 |
| PII | S0308-8146(17)31019-1 |
| PMID | 28764098 |
| MeSH | Cloning, Molecular Enzyme Stability Polygalacturonase Talaromyces / enzymology* Temperature Vitis |
| IF | 6.306 |
| Times Cited | 8 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM 12802 |