| RRC ID |
50962
|
| 著者 |
Romaniuk K, Krucon T, Decewicz P, Gorecki A, Dziewit L.
|
| タイトル |
Molecular characterization of the pA3J1 plasmid from the psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_J3.
|
| ジャーナル |
Plasmid
|
| Abstract |
The knowledge on plasmids of cold-active bacteria is highly limited. In this study, the molecular characterization of the pA3J1 plasmid of Antarctic psychrotolerant bacterium Pseudomonas sp. ANT_J3 was performed. Within this plasmid, thirteen putative open reading frames were identified. Nine of them encoded proteins involved in replication, partitioning, postsegregational elimination of plasmid-less cells (via a toxin-antitoxin system activity), multimer resolution and mobilization by conjugal transfer. These genes constitute the plasmid backbone. The functional analysis of the pA3J1 maintenance region revealed that it is a narrow host range replicon, stably maintained in the host cells by the combined activities of the partitioning and relBE-type toxin-antitoxin systems. It was also suggested that the replication system of the pA3J1 plasmid may be temperature-sensitive. Comparative analyses revealed the presence of 16 Pseudomonas plasmids encoding homologous replication proteins and 5 plasmids carrying mobA genes homologous to the corresponding gene of pA3J1. The relaxase (MobA) of the pA3J1 plasmid was classified into MOBQ family, and the phylogenetic analysis suggested that this may be a representative of a novel group (or subgroup) within this family. The structural and comparative analyses revealed that the arrangement of genetic modules in the pA3J1 plasmid is unique.
|
| 巻・号 |
92
|
| ページ |
49-56
|
| 公開日 |
2017-7-1
|
| DOI |
10.1016/j.plasmid.2017.08.001
|
| PII |
S0147-619X(17)30021-5
|
| PMID |
28802584
|
| MeSH |
Amino Acid Sequence
Antarctic Regions
Bacterial Proteins / chemistry
Bacterial Proteins / genetics
DNA Replication / genetics
Environmental Microbiology
Gene Transfer, Horizontal
Open Reading Frames
Phylogeny
Plasmids / genetics*
Pseudomonas / genetics*
Replicon
Sequence Analysis, DNA
|
| IF |
2.805
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM 7364 |
