| RRC ID |
50968
|
| Author |
Katoh T, Maeshibu T, Kikkawa KI, Gotoh A, Tomabechi Y, Nakamura M, Liao WH, Yamaguchi M, Ashida H, Yamamoto K, Katayama T.
|
| Title |
Identification and characterization of a sulfoglycosidase from Bifidobacterium bifidum implicated in mucin glycan utilization.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Human gut symbiont bifidobacteria possess carbohydrate-degrading enzymes that act on the O-linked glycans of intestinal mucins to utilize those carbohydrates as carbon sources. However, our knowledge about mucin type O-glycan degradation by bifidobacteria remains fragmentary, especially regarding how they decompose sulfated glycans, which are abundantly found in mucin sugar-chains. Here, we examined the abilities of several Bifidobacterium strains to degrade a sulfated glycan substrate and identified a 6-sulfo-β-d-N-acetylglucosaminidase, also termed sulfoglycosidase, encoded by bbhII from Bifidobacterium bifidum JCM 7004. A recombinant BbhII protein showed a substrate preference toward 6-sulfated and 3,4-disulfated N-acetylglucosamines over non-sulfated and 3-sulfated N-acetylglucosamines. The purified BbhII directly released 6-sulfated N-acetylglucosamine from porcine gastric mucin and the expression of bbhII was moderately induced in the presence of mucin. This de-capping activity may promote utilization of sulfated glycans of mucin by other bacteria including bifidobacteria, thereby establishing the symbiotic relationship between human and gut microbes.
|
| Volume |
81(10)
|
| Pages |
2018-2027
|
| Published |
2017-10-1
|
| DOI |
10.1080/09168451.2017.1361810
|
| PMID |
28814130
|
| MeSH |
Acetylglucosaminidase / chemistry
Acetylglucosaminidase / genetics
Acetylglucosaminidase / metabolism*
Amino Acid Sequence
Bifidobacterium bifidum / enzymology*
Bifidobacterium bifidum / genetics
Bifidobacterium bifidum / metabolism
Gene Expression Regulation, Bacterial
Mucins / metabolism*
Polysaccharides / metabolism*
|
| IF |
1.516
|
| Times Cited |
9
|
| Resource |
| General Microbes |
JCM 7004
JCM 1254 |
