論文 - 詳細
| RRC ID | 50985 |
|---|---|
| 著者 | Kim MM, Siddiqi MZ, Im WT. |
| タイトル | Mucilaginibacter ginsenosidivorans sp. nov., Isolated from Soil of Ginseng Field. |
| ジャーナル | Curr Microbiol |
| Abstract |
A Gram-reaction-negative, aerobic, nonmotile, nonspore-forming, and rod-shaped bacterial strain designated Gsoil 3017T was isolated from soil of ginseng field and investigated by phenotypic and phylogenetic analyses. Strain Gsoil 3017T grew at 10-37 °C (optimal growth at 30 °C) and at pH 5.5-8.0 (optimal growth at pH 7) on R2A and nutrient agar without additional NaCl as a supplement. Strain Gsoil 3017T possessed β-glucosidase activity, which was responsible for its ability to transform ginsenosides Rb1, Rc, and Rd (the three dominant active components of ginseng) to F2 and C-K, respectively. Based on 16S rRNA gene phylogeny, the novel strain represents a new branch within the genus Mucilaginibacter family Sphingobacteriaceae, and clusters with Mucilaginibacter frigoritolerans FT22T (95.6%) and Mucilaginibacter gotjawali SA3-7T (95.6%). The G+C content of the genomic DNA was 48.7%. The predominant respiratory quinone was MK-7, and the major fatty acids were iso-C15:0, iso-C17:0 3-OH, and summed feature 3 (comprising C16:1 ω6c and/or C16:1 ω7c). The major polar lipid was phosphatidylethanolamine. Strain Gsoil 3017T could be differentiated genotypically and phenotypically from other type strains of the genus Mucilaginibacter. The isolate therefore represents a novel species, for which the name Mucilaginibacter ginsenosidivorans sp. nov. is proposed, with the type strain Gsoil 3017T (=KACC 14954T = JCM 17081T). |
| 巻・号 | 74(12) |
| ページ | 1382-1388 |
| 公開日 | 2017-12-1 |
| DOI | 10.1007/s00284-017-1329-4 |
| PII | 10.1007/s00284-017-1329-4 |
| PMID | 28821948 |
| MeSH | Aerobiosis Bacterial Typing Techniques Bacteroidetes / classification* Bacteroidetes / genetics Bacteroidetes / isolation & purification* Bacteroidetes / physiology Base Composition Cluster Analysis Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Hydrogen-Ion Concentration Panax / growth & development Phospholipids / analysis Phylogeny Quinones / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Soil Microbiology* Temperature beta-Glucosidase / metabolism |
| IF | 1.746 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 一般微生物 | JCM 17081 |