RRC ID 5100
著者 Ben Dhia Thabet O, Fardeau ML, Suarez-Nuñez C, Hamdi M, Thomas P, Ollivier B, Alazard D.
タイトル Desulfovibrio marinus sp. nov., a moderately halophilic sulfate-reducing bacterium isolated from marine sediments in Tunisia.
ジャーナル Int J Syst Evol Microbiol
Abstract Two novel sulfate-reducing bacterial strains, designated E-2(T) and IMP-2, were isolated from geographically distinct locations. Strain E-2(T) was recovered from marine sediments near Sfax (Tunisia), whereas strain IMP-2 originated from oilfield production fluids in the Gulf of Mexico. Cells were Gram-negative, non-sporulated, motile, vibrio-shaped or sigmoid. They were strictly anaerobic, mesophilic and moderately halophilic. Sulfate, sulfite, thiosulfate and elemental sulfur served as electron acceptors, but not nitrate or nitrite. H(2) (with acetate as carbon source), formate, fumarate, lactate, malate, pyruvate, succinate and fructose were used as electron donors in the presence of sulfate as terminal electron acceptor. Lactate was oxidized incompletely to acetate. Fumarate and pyruvate were fermented. Desulfoviridin and c-type cytochromes were present. 16S rRNA gene sequence analysis of the two strains showed that they were phylogenetically similar (99.0 % similarity) and belonged to the genus Desulfovibrio, with Desulfovibrio indonesiensis and Desulfovibrio gabonensis as their closest phylogenetic relatives. The G+C content of the DNA was respectively 60.4 and 62.7 mol% for strains E-2(T) and IMP-2. DNA-DNA hybridization experiments revealed that the novel strains had a high genomic relatedness, suggesting that they belong to the same species. We therefore propose that the two isolates be affiliated to a novel species of the genus Desulfovibrio, Desulfovibrio marinus sp. nov. The type strain is strain E-2(T) (=DSM 18311(T) =JCM 14040(T)).
巻・号 57(Pt 9)
ページ 2167-2170
公開日 2007-9-1
DOI 10.1099/ijs.0.64790-0
PMID 17766893
MeSH Anaerobiosis / physiology Bacterial Typing Techniques Base Composition Cytochromes c / analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Desulfovibrio / classification* Desulfovibrio / genetics Desulfovibrio / isolation & purification* Desulfovibrio / physiology Fermentation / physiology Genes, rRNA Geologic Sediments / microbiology* Hydrogensulfite Reductase / analysis Locomotion / physiology Mexico Molecular Sequence Data Nucleic Acid Hybridization Oxidation-Reduction Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Homology, Nucleic Acid Sulfates / metabolism Temperature Tunisia Water Microbiology*
IF 2.415
引用数 13
リソース情報
一般微生物 JCM14040