| RRC ID |
51001
|
| 著者 |
Kajihara Y, Yoshikawa S, Cho Y, Ito T, Miyamoto H, Kodama H.
|
| タイトル |
Preferential isolation of Megasphaera elsdenii from pig feces.
|
| ジャーナル |
Anaerobe
|
| Abstract |
Lactic acid produced by intestinal bacteria is fermented by lactate-utilizing bacteria. In this study, we developed a selective culture medium (KMI medium) for Megasphaera elsdenii, a lactate-utilizing bacterium that is abundant in pig intestines. Supplementation of the medium with lactate and beef extract powder was necessary for the preferential growth of M. elsdenii. In addition, we designed a species-specific primer set to detect M. elsdenii. When pig fecal samples were plated on KMI agar medium, approximately 60-100% of the resulting colonies tested positive using the M. elsdenii-specific PCR primers. In fact, nearly all of the large, yellow-white colonies that grew on the KMI agar medium tested positive by PCR with this primer set. The 16S rRNA gene sequences of three representative PCR-positive strains showed strong similarities to that of M. elsdenii ATCC 25940T (98.9-99.2% identity). These three strains were approximately 1.5 μm sized cocci that were primarily arranged in pairs, as was observed for M. elsdenii JCM 1772T. The selective KMI medium and species-specific primer set developed in this study are useful for the isolation and detection of M. elsdenii and will be useful in research aimed at increasing our understanding of intestinal short-chain fatty acid metabolism in pigs.
|
| 巻・号 |
48
|
| ページ |
160-164
|
| 公開日 |
2017-12-1
|
| DOI |
10.1016/j.anaerobe.2017.08.013
|
| PII |
S1075-9964(17)30172-5
|
| PMID |
28842275
|
| MeSH |
Animals
Feces / microbiology*
Megasphaera elsdenii / classification
Megasphaera elsdenii / genetics
Megasphaera elsdenii / isolation & purification*
Megasphaera elsdenii / ultrastructure
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Swine
|
| IF |
2.709
|
| 引用数 |
2
|
| リソース情報 |
| 一般微生物 |
JCM 1772 |
