論文 - 詳細
| RRC ID | 5111 |
|---|---|
| 著者 | Takii S, Hanada S, Tamaki H, Ueno Y, Sekiguchi Y, Ibe A, Matsuura K. |
| タイトル | Dethiosulfatibacter aminovorans gen. nov., sp. nov., a novel thiosulfate-reducing bacterium isolated from coastal marine sediment via sulfate-reducing enrichment with Casamino acids. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A sulfate-reducing enrichment culture originating from coastal marine sediment of the eutrophic Tokyo Bay, Japan, was successfully established with Casamino acids as a substrate. A thiosulfate reducer, strain C/G2(T), was isolated from the enrichment culture after further enrichment with glutamate. Cells of strain C/G2(T) were non-motile rods (0.6-0.8 microm x 2.2-4.8 microm) and were found singly or in pairs and sometimes in short chains. Spores were not formed. Cells of strain C/G2(T) stained Gram-negatively, despite possessing Gram-positive cell walls. The optimum temperature for growth was 28-30 degrees C, the optimum pH was around 7.8 and the optimum salt concentration was 20-30 g l(-1). Lactate, pyruvate, serine, cysteine, threonine, glutamate, histidine, lysine, arginine, Casamino acids, peptone and yeast extract were fermented as single substrates and no sugar was used as a fermentative substrate. A Stickland reaction was observed with some pairs of amino acids. Fumarate, alanine, proline, phenylalanine, tryptophan, glutamine and aspartate were utilized only in the presence of thiosulfate. Strain C/G2(T) fermented glutamate to H2, CO2, acetate and propionate. Thiosulfate and elemental sulfur were reduced to sulfide. Sulfate, sulfite and nitrate were not utilized as electron acceptors. The growth of strain C/G2(T) on Casamino acids or glutamate was enhanced by co-culturing with Desulfovibrio sp. isolated from the original mixed culture enriched with Casamino acids. The DNA G+C content of strain C/G2(T) was 41.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain C/G2(T) formed a distinct cluster with species of the genus Sedimentibacter. The closest relative was Sedimentibacter hydroxybenzoicus (with a gene sequence similarity of 91 %). On the basis of its phylogenetic and phenotypic properties, strain C/G2(T) (=JCM 13356(T)=NBRC 101112(T)=DSM 17477(T)) is proposed as representing a new genus and novel species, Dethiosulfatibacter aminovorans gen. nov., sp. nov. |
| 巻・号 | 57(Pt 10) |
| ページ | 2320-2326 |
| 公開日 | 2007-10-1 |
| DOI | 10.1099/ijs.0.64882-0 |
| PMID | 17911304 |
| MeSH | Bacterial Typing Techniques Bacteriological Techniques / methods* Base Composition Carbon / metabolism Chlorates / metabolism Culture Media / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fermentation / physiology Genes, rRNA Geologic Sediments / microbiology* Glutamic Acid / metabolism Gram-Positive Bacteria / classification* Gram-Positive Bacteria / cytology Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / physiology Hydrogen-Ion Concentration Japan Molecular Sequence Data Nitrogen / metabolism Oxidation-Reduction Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Temperature Thiosulfates / metabolism* |
| IF | 2.415 |
| 引用数 | 20 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM13356 |