RRC ID 51124
Author Taniguchi M, Kawabe J, Toyoda R, Namae T, Ochiai A, Saitoh E, Tanaka T.
Title Cationic peptides from peptic hydrolysates of rice endosperm protein exhibit antimicrobial, LPS-neutralizing, and angiogenic activities.
Journal Peptides
Abstract In this study, we hydrolyzed rice endosperm protein (REP) with pepsin and generated 20 fractions containing multifunctional cationic peptides with varying isoelectric point (pI) values using ampholyte-free isoelectric focusing (autofocusing). Subsequently, we determined antimicrobial activities of each fraction against the pathogens Prophyromonas gingivalis, Propionibacterium acnes, Streptocossus mutans, and Candida albicans. Fractions 18, 19, and 20 had pI values greater than 12 and exhibited antimicrobial activity against P. gingivalis, P. acnes, and C. albicans, but not against S. mutans. In further experiments, we purified and identified cationic peptides from fractions 18, 19, and 20 using reversed-phase high-performance liquid chromatography and matrix-assisted laser/desorption ionization-time-of-flight mass spectroscopy. We also chemically synthesized five identified peptides (RSVSKSR, RRVIEPR, ERFQPMFRRPG, RVRQNIDNPNRADTYNPRAG, and VVRRVIEPRGLL) with pI values greater than 10.5 and evaluated antimicrobial, lipopolysaccharide (LPS)-neutralizing, and angiogenic activities. Among these synthetic peptides, only VVRRVIEPRGLL exhibited antimicrobial activity against P. gingivalis, with an IC50 value of 87μM. However, all five cationic peptides exhibited LPS-neutralizing and angiogenic activities with little or no hemolytic activity against mammalian red blood cells at functional concentrations. These present data show dual or multiple functions of the five identified cationic peptides with little or no hemolytic activity. Therefore, fractions containing cationic peptides from REP hydrolysates have the potential to be used as dietary supplements and functional ingredients in food products.
Volume 97
Pages 70-78
Published 2017-11
DOI 10.1016/j.peptides.2017.09.019
PII S0196-9781(17)30302-9
PMID 28987278
MeSH Angiogenic Proteins / chemistry* Angiogenic Proteins / pharmacology Animals Anti-Infective Agents / chemistry* Anti-Infective Agents / pharmacology Antifungal Agents / pharmacology Antimicrobial Cationic Peptides / chemical synthesis Antimicrobial Cationic Peptides / chemistry* Bacteria / drug effects Endosperm / chemistry* Fungi / drug effects Hemolysis / drug effects Human Umbilical Vein Endothelial Cells Humans Hydrolysis Inhibitory Concentration 50 Lipopolysaccharides Oryza / chemistry* Plant Proteins / chemistry*
IF 2.851
Resource
General Microbes JCM 6473 JCM 5705