RRC ID |
51156
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著者 |
Saika A, Koike H, Yamamoto S, Kishimoto T, Morita T.
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タイトル |
Enhanced production of a diastereomer type of mannosylerythritol lipid-B by the basidiomycetous yeast Pseudozyma tsukubaensis expressing lipase genes from Pseudozyma antarctica.
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ジャーナル |
Appl Microbiol Biotechnol
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Abstract |
Basidiomycetous yeasts in the genus Pseudozyma are known to produce extracellular glycolipids called mannosylerythritol lipids (MELs). Pseudozyma tsukubaensis produces a large amount of MEL-B using olive oil as the sole carbon source (> 70 g/L production). The MEL-B produced by P. tsukubaensis is a diastereomer type of MEL-B, which consists of 4-O-β-D-mannopyranosyl-(2R,3S)-erythritol as a sugar moiety, in contrast to the conventional type of MELs produced by P. antarctica, which contain 4-O-β-D mannopyranosyl-(2S,3R)-erythritol. In this study, we attempted to increase the production of the diastereomer type of MEL-B in P. tsukubaensis 1E5 by introducing the genes encoding two lipases, PaLIPAp (PaLIPA) and PaLIPBp (PaLIPB) from P. antarctica T-34. Strain 1E5 expressing PaLIPA exhibited higher lipase activity than the strain possessing an empty vector, which was used as a negative control. Strains of 1E5 expressing PaLIPA or PaLIPB showed 1.9- and 1.6-fold higher MEL-B production than the negative control strain, respectively, and oil consumption was also accelerated by the introduction of these lipase genes. MEL-B production was estimated using time course analysis in the recombinant strains. Strain 1E5 expressing PaLIPA produced 37.0 ± 1.2 g/L of MEL-B within 4 days of cultivation, whereas the strain expressing an empty vector produced 22.1 ± 7.5 g/L in this time. Overexpression of PaLIPA increased MEL-B production by P. tsukubaensis strain 1E5 from olive oil as carbon source by more than 1.7-fold.
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巻・号 |
101(23-24)
|
ページ |
8345-8352
|
公開日 |
2017-12-1
|
DOI |
10.1007/s00253-017-8589-6
|
PII |
10.1007/s00253-017-8589-6
|
PMID |
29075829
|
MeSH |
Glycolipids / biosynthesis*
Lipase / genetics
Lipase / metabolism*
Metabolic Engineering*
Olive Oil / metabolism
Recombinant Proteins / genetics
Recombinant Proteins / metabolism*
Ustilaginales / enzymology*
Ustilaginales / genetics
Ustilaginales / metabolism*
|
IF |
3.53
|
引用数 |
4
|
リソース情報 |
一般微生物 |
JCM 16987 |