RRC ID 51156
著者 Saika A, Koike H, Yamamoto S, Kishimoto T, Morita T.
タイトル Enhanced production of a diastereomer type of mannosylerythritol lipid-B by the basidiomycetous yeast Pseudozyma tsukubaensis expressing lipase genes from Pseudozyma antarctica.
ジャーナル Appl Microbiol Biotechnol
Abstract Basidiomycetous yeasts in the genus Pseudozyma are known to produce extracellular glycolipids called mannosylerythritol lipids (MELs). Pseudozyma tsukubaensis produces a large amount of MEL-B using olive oil as the sole carbon source (> 70 g/L production). The MEL-B produced by P. tsukubaensis is a diastereomer type of MEL-B, which consists of 4-O-β-D-mannopyranosyl-(2R,3S)-erythritol as a sugar moiety, in contrast to the conventional type of MELs produced by P. antarctica, which contain 4-O-β-D mannopyranosyl-(2S,3R)-erythritol. In this study, we attempted to increase the production of the diastereomer type of MEL-B in P. tsukubaensis 1E5 by introducing the genes encoding two lipases, PaLIPAp (PaLIPA) and PaLIPBp (PaLIPB) from P. antarctica T-34. Strain 1E5 expressing PaLIPA exhibited higher lipase activity than the strain possessing an empty vector, which was used as a negative control. Strains of 1E5 expressing PaLIPA or PaLIPB showed 1.9- and 1.6-fold higher MEL-B production than the negative control strain, respectively, and oil consumption was also accelerated by the introduction of these lipase genes. MEL-B production was estimated using time course analysis in the recombinant strains. Strain 1E5 expressing PaLIPA produced 37.0 ± 1.2 g/L of MEL-B within 4 days of cultivation, whereas the strain expressing an empty vector produced 22.1 ± 7.5 g/L in this time. Overexpression of PaLIPA increased MEL-B production by P. tsukubaensis strain 1E5 from olive oil as carbon source by more than 1.7-fold.
巻・号 101(23-24)
ページ 8345-8352
公開日 2017-12-1
DOI 10.1007/s00253-017-8589-6
PII 10.1007/s00253-017-8589-6
PMID 29075829
MeSH Glycolipids / biosynthesis* Lipase / genetics Lipase / metabolism* Metabolic Engineering* Olive Oil / metabolism Recombinant Proteins / genetics Recombinant Proteins / metabolism* Ustilaginales / enzymology* Ustilaginales / genetics Ustilaginales / metabolism*
IF 3.53
引用数 4
リソース情報
一般微生物 JCM 16987