RRC ID 51233
著者 Takahashi Y, Ono K, Akamine Y, Asano T, Ezaki M, Mouri I.
タイトル Highly-expressed polyamine oxidases catalyze polyamine back conversion in Brachypodium distachyon.
ジャーナル J Plant Res
Abstract To understand the polyamine (PA) catabolic pathways in Brachypodium distachyon, we focused on the flavin-containing polyamine oxidase enzymes (PAO), and characterized them at the molecular and biochemical levels. Five PAO isoforms were identified from database searches, and we named them BdPAO1 to BdPAO5. By gene expression analysis using above-ground tissues such as leaf, stem and inflorescence, it was revealed that BdPAO2 is the most abundant PAO gene in normal growth conditions, followed by BdPAO3 and BdPAO4. BdPAO1 and BdPAO5 were expressed at very low levels. All Arabidopsis thaliana and rice orthologs belonging to the same clade as BdPAO2, BdPAO3 and BdPAO4 have conserved peroxisome-targeting signal sequences at their C-termini. Amino acid sequences of BdPAO2 and BdPAO4 also showed such a sequence, but BdPAO3 did not. We selected the gene with the highest expression level (BdPAO2) and the peroxisome-targeting signal lacking PAO (BdPAO3) for biochemical analysis of substrate specificity and catabolic pathways. BdPAO2 catalyzed conversion of spermine (Spm) or thermospermine to spermidine (Spd), and Spd to putrescine, but its most-favored substrate was Spd. In contrast, BdPAO3 favored Spm as substrate and catalyzed conversion of tetraamines to Spd. These results indicated that the major PAOs in B. distachyon have back-conversion activity.
巻・号 131(2)
ページ 341-348
公開日 2018-3-1
DOI 10.1007/s10265-017-0989-2
PII 10.1007/s10265-017-0989-2
PMID 29063977
MeSH Amino Acid Sequence Brachypodium / genetics* Gene Expression* Metabolic Networks and Pathways / genetics Oxidoreductases Acting on CH-NH Group Donors / chemistry Oxidoreductases Acting on CH-NH Group Donors / genetics* Oxidoreductases Acting on CH-NH Group Donors / metabolism Phylogeny Plant Proteins / chemistry Plant Proteins / genetics* Plant Proteins / metabolism Polyamines / metabolism* Sequence Alignment
IF 2.0
引用数 4
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 psb00001