RRC ID 51258
Author Horstick EJ, Bayleyen Y, Sinclair JL, Burgess HA.
Title Search strategy is regulated by somatostatin signaling and deep brain photoreceptors in zebrafish.
Journal BMC Biol
Abstract BACKGROUND:Animals use sensory cues to efficiently locate resources, but when sensory information is insufficient, they may rely on internally coded search strategies. Despite the importance of search behavior, there is limited understanding of the underlying neural mechanisms in vertebrates.
RESULTS:Here, we report that loss of illumination initiates sophisticated light-search behavior in larval zebrafish. Using three-dimensional tracking, we show that at the onset of darkness larvae swim in a helical trajectory that is spatially restricted in the horizontal plane, before gradually transitioning to an outward movement profile. Local and outward swim patterns display characteristic features of area-restricted and roaming search strategies, differentially enhancing phototaxis to nearby and remote sources of light. Retinal signaling is only required to initiate area-restricted search, implying that photoreceptors within the brain drive the transition to the roaming search state. Supporting this, orthopediaA mutant larvae manifest impaired transition to roaming search, a phenotype which is recapitulated by loss of the non-visual opsin opn4a and somatostatin signaling.
CONCLUSION:These findings define distinct neuronal pathways for area-restricted and roaming search behaviors and clarify how internal drives promote goal-directed activity.
Volume 15(1)
Pages 4
Published 2017-1-26
DOI 10.1186/s12915-016-0346-2
PII 10.1186/s12915-016-0346-2
PMID 28122559
PMC PMC5267475
MeSH Animals Behavior, Animal* Brain / metabolism* Imaging, Three-Dimensional Lighting Locomotion* Models, Biological Neurons / metabolism Photoreceptor Cells, Vertebrate / metabolism* Retinaldehyde / metabolism Rod Opsins / metabolism Signal Transduction* Somatostatin / metabolism* Swimming Zebrafish / metabolism*
IF 6.765
Times Cited 15
Zebrafish Tg(vglut2a:loxP-DsRed-loxP-GFP)